Protein Info for LRK54_RS02855 in Rhodanobacter denitrificans FW104-10B01

Annotation: type I restriction-modification system subunit M

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 539 TIGR00497: type I restriction-modification system, M subunit" amino acids 7 to 518 (512 residues), 377.4 bits, see alignment E=5.6e-117 PF12161: HsdM_N" amino acids 9 to 170 (162 residues), 85.2 bits, see alignment E=7.2e-28 PF02384: N6_Mtase" amino acids 180 to 500 (321 residues), 351.9 bits, see alignment E=3.5e-109

Best Hits

KEGG orthology group: K03427, type I restriction enzyme M protein [EC: 2.1.1.72] (inferred from 84% identity to xcv:XCV0513)

Predicted SEED Role

"Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)" in subsystem Restriction-Modification System (EC 2.1.1.72)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (539 amino acids)

>LRK54_RS02855 type I restriction-modification system subunit M (Rhodanobacter denitrificans FW104-10B01)
MTENDQKQLGKTLWNVADQLRGTMDADDFRDYMLSFLFLRYLSDNYEAAARKELGSDYPD
PNAIGNAGKTPLSVWYAANKADVAQFEQQMRRKAHYVIQPKYLWGHIVHLAKTQDDTLLD
TLQGGFKYIEEESFQSHFQGLFSEINLGSDKLGRKYHDRNAKLCSIISEIARGMALFSTD
SDTLGDAYEYLIGQFAAGSGKKAGEFYTPQQISNILSAIITLDSQEPKTGPRRKLDSVFD
FACGSGSLLLNVRHRMTEAGGRIGRIYGQESNITTYNLARMNMLLHGVKDTEFEIWHGDT
LKNDWDWLRETNPAKKPKFDAVVANPPFSYRWEPKDGTKDDPRFRNHGVAPKSAADFAFL
LHGLHYLKDDGVMAIILPHGVLFRGGKEADIRTKLLTDGHIDTVIGLPANLFYSTGIPVC
ILVLKKCKKPDDVLFINAADHFVKGKRQNQLTEEHIARIVSTYQNRDEHERYSKRVSMKR
IAEEGYNLNISRYVSTAEAETAIDLKTVHADLEAIEAKIDEAKKRHNAFLKELGLAPLP