Protein Info for LRK54_RS02645 in Rhodanobacter denitrificans FW104-10B01

Annotation: S-formylglutathione hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02821: S-formylglutathione hydrolase
  • amino acids 5 to 279 (275 residues), 453.1 bits, E=1.9e-140
• PF00756: Esterase
  • amino acids 24 to 273 (250 residues), 180.2 bits, E=3e-57

Best Hits

Swiss-Prot: 57% identical to SFGH_PARDP: S-formylglutathione hydrolase (fghA) from Paracoccus denitrificans (strain Pd 1222)

KEGG orthology group: K01070, S-formylglutathione hydrolase [EC: 3.1.2.12] (inferred from 63% identity to bur:Bcep18194_A5934)

MetaCyc: 53% identical to S-formylglutathione hydrolase / S-lactoylglutathione hydrolase (Escherichia coli K-12 substr. MG1655)
Hydroxyacylglutathione hydrolase. [EC: 3.1.2.6]; S-formylglutathione hydrolase. [EC: 3.1.2.6, 3.1.2.12]

Predicted SEED Role

"S-formylglutathione hydrolase (EC 3.1.2.12)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 3.1.2.12)

MetaCyc Pathways

• formaldehyde oxidation II (glutathione-dependent) (3/3 steps found)
• methylglyoxal degradation I (2/3 steps found)
• methylglyoxal degradation VIII (2/3 steps found)
• superpathway of methylglyoxal degradation (4/8 steps found)
• superpathway of C1 compounds oxidation to CO₂ (3/12 steps found)

KEGG Metabolic Maps

• Methane metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.6

Use Curated BLAST to search for 3.1.2.12 or 3.1.2.6

Protein Sequence (282 amino acids)

>LRK54_RS02645 S-formylglutathione hydrolase (Rhodanobacter denitrificans FW104-10B01)
MGMLETISEQRCHGGIQGFYRHHSEACAGAMRFAVYQPPQAGRQLCPVLYFLAGLTCTEE
TATIKAGAQRLAAELGLVLVMPDTSPRGTGIDGATDDWEFGEGAGFYLDATEAPWSARFR
MASYVVDELPALIGAQFPVDAALSGICGHSMGGHGALTLALKHPQRYRSVSAFAPIVAPS
QVPWGRKALPRYLGDDPAAWADHDACELVRRQTFPGSILIDQGEADGFLEAQLKPELFDQ
ACAEAGQPLLLRRHAGYDHSYWFIASFIDDHLRHHAAVLGLL