Protein Info for LRK54_RS02250 in Rhodanobacter denitrificans FW104-10B01

Annotation: murein biosynthesis integral membrane protein MurJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 536 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 34 to 48 (15 residues), see Phobius details amino acids 92 to 113 (22 residues), see Phobius details amino acids 135 to 154 (20 residues), see Phobius details amino acids 162 to 182 (21 residues), see Phobius details amino acids 188 to 211 (24 residues), see Phobius details amino acids 237 to 262 (26 residues), see Phobius details amino acids 274 to 294 (21 residues), see Phobius details amino acids 316 to 339 (24 residues), see Phobius details amino acids 353 to 377 (25 residues), see Phobius details amino acids 390 to 412 (23 residues), see Phobius details amino acids 432 to 452 (21 residues), see Phobius details amino acids 471 to 492 (22 residues), see Phobius details amino acids 504 to 527 (24 residues), see Phobius details TIGR01695: murein biosynthesis integral membrane protein MurJ" amino acids 6 to 407 (402 residues), 400.7 bits, see alignment E=4.8e-124 PF03023: MurJ" amino acids 31 to 406 (376 residues), 401.7 bits, see alignment E=3.8e-124 PF13440: Polysacc_synt_3" amino acids 193 to 340 (148 residues), 35.2 bits, see alignment E=8.4e-13

Best Hits

KEGG orthology group: K03980, virulence factor (inferred from 76% identity to xal:XALc_0920)

Predicted SEED Role

"Proposed peptidoglycan lipid II flippase MurJ" in subsystem ZZ gjo need homes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (536 amino acids)

>LRK54_RS02250 murein biosynthesis integral membrane protein MurJ (Rhodanobacter denitrificans FW104-10B01)
MKSPSMLRGLLSFSSMTMVSRVLGLVRDMSINAAFGANGATDAFWVAFRIPNFMRRLFAE
GSFSTAFVPVFTEVKEKGSHAQLKELMARVSGTLGGVLLLITALGILFAPQVTVLFSPGA
IDEPRKFELTVELLRLTFPFLLFVSLTALSGGALNSFHRFGLPALTPVILNLCMIAGALW
LSKWLQTPIMAMGWAILAAGILQLLFQLPALRQLDLLTLPRWGWSHPEVRKIMRLMVPTL
FGSSVAQINLLFDTVIASLLVVGSQSWLSQADRFLELPLGVFGVALGTVILPSLSRHHVS
TDTAGFSRALDWGLRVTLLIAVPAMFALMLLAGPLVATLFQHGHWSAHDTAMATLSITAL
SFGLPAFALVKVLLPAFYARQDTRTPVRAAVASLLANMLLNLVFLALLFELWAPVELKRL
PWLDGLARLPGLHMALGLASAVAAYVNLGLLWHWLKKAGVYQRQPGWSRHLLRLAAACTV
MVTVLLLGRWLWPDWTHGVSVLTRLWHLTVLVLAGGASYVAVLFAGGFRLRDLRAA