Protein Info for LRK54_RS02245 in Rhodanobacter denitrificans FW104-10B01

Annotation: bifunctional riboflavin kinase/FAD synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06574: FAD_syn
  • amino acids 15 to 166 (152 residues), 155.5 bits, E=1e-49
• TIGR00083: riboflavin biosynthesis protein RibF
  • amino acids 20 to 308 (289 residues), 267.7 bits, E=6.8e-84
• PF01687: Flavokinase
  • amino acids 184 to 308 (125 residues), 133.3 bits, E=5.7e-43

Best Hits

Swiss-Prot: 48% identical to RIBF_PSEFL: Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF) from Pseudomonas fluorescens

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 56% identity to xal:XALc_0921)

MetaCyc: 44% identical to bifunctional riboflavin kinase / FMN adenylyltransferase (Escherichia coli K-12 substr. MG1655)
FAD synthetase. [EC: 2.7.7.2]; Riboflavin kinase. [EC: 2.7.7.2, 2.7.1.26]

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• flavin salvage (2/2 steps found)
• flavin biosynthesis III (fungi) (7/9 steps found)
• 5,6-dimethylbenzimidazole biosynthesis I (aerobic) (1/3 steps found)
• flavin biosynthesis II (archaea) (5/10 steps found)
• roseoflavin biosynthesis (1/7 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (12/33 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Protein Sequence (317 amino acids)

>LRK54_RS02245 bifunctional riboflavin kinase/FAD synthetase (Rhodanobacter denitrificans FW104-10B01)
MMRLSRDVAGPCLAPRGSVIAVGAFDGLHRGHQALLAQVRERAQALGCSPAVVSFEPLPR
AFFSPEPVPRLSSVREKLRGFAAAGIEETLLLRFNRALTAMSAEAFVRRVLVERLAAREV
WVGGDFRFGHGRAGDVALLERMGAQFGFRACAMPTIQLDGTRVSASRVRALLGAGEFAGA
EPLLGRPFVIDGKVEYGNQLGRMLGYPTANIHLQQRVSPIQGIFAVRVGLGEGECSWPGV
ASLGVRPTVNEVSQPLLEVHLFDFEGDLYGQRMAVQFVAKLRDEQKFDGLEPLKAQMALD
ARRARELLGMNPRLAEA