Protein Info for LRK54_RS01650 in Rhodanobacter denitrificans FW104-10B01

Annotation: glutamate--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00464: glutamate--tRNA ligase
  • amino acids 2 to 459 (458 residues), 569.6 bits, E=2.8e-175
• PF00749: tRNA-synt_1c
  • amino acids 3 to 305 (303 residues), 341.5 bits, E=3.9e-106
• PF19269: Anticodon_2
  • amino acids 342 to 461 (120 residues), 101.8 bits, E=4.7e-33

Best Hits

Swiss-Prot: 68% identical to SYE_XANCB: Glutamate--tRNA ligase (gltX) from Xanthomonas campestris pv. campestris (strain B100)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 68% identity to xca:xccb100_1475)

MetaCyc: 54% identical to glutamate--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glutamate--tRNA ligase. [EC: 6.1.1.17]

Predicted SEED Role

"Glutamyl-tRNA synthetase (EC 6.1.1.17)" in subsystem Heme and Siroheme Biosynthesis or tRNA aminoacylation, Glu and Gln (EC 6.1.1.17)

MetaCyc Pathways

• tRNA charging (21/21 steps found)
• superpathway of heme b biosynthesis from glutamate (10/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (12/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (12/36 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Glutamate metabolism
• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.17

Protein Sequence (466 amino acids)

>LRK54_RS01650 glutamate--tRNA ligase (Rhodanobacter denitrificans FW104-10B01)
MTVRTRFAPSPTGFLHIGGARTALYCWLEARRRGGEFILRIEDTDRERSTEEAVQAILDA
MHWLDLDADEGPFYQTQRLARYRQVADELVAAGKAYYAYESKEEIEAMREAAMKVGEKPR
YNGYYRDRNEPFRDDPNRVIRFRNPTEGSVVFEDKVKGRIEIANSELDDLVIFRSDGWPT
YNFAVVVDDIDMGITEVIRGDDHVNNTPRQINIYQALGAAVPEFAHLPMILDKEGKKLSK
RTNSVSVMEYRAAGFLPHAILNYLVRLGWSHGDQEIFSRGEMIALFDIADVNKAASRFDT
EKLAWLNQHYLKTDEPQSLAAEFAWHLQRAGIDASRGPAPVDVVVALRDRVHTLKEMAER
AKIWYGPIVEWDDKAVAKHLKNDSAVAVLEVAKALLAEVEWQPAPIHQVIEQVAAQLALG
MGKVAQPLRVAMTGTQVSPSIEHTIYLAGRDEALTRIDAAIAMARE