Protein Info for LRK54_RS01065 in Rhodanobacter denitrificans FW104-10B01

Annotation: NADH-quinone oxidoreductase subunit NuoN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01770: proton-translocating NADH-quinone oxidoreductase, chain N
  • amino acids 8 to 470 (463 residues), 473.1 bits, E=5.8e-146
• PF19530: Ndh2_N
  • amino acids 9 to 83 (75 residues), 27.6 bits, E=2.9e-10
• PF00361: Proton_antipo_M
  • amino acids 124 to 422 (299 residues), 254.1 bits, E=1.7e-79

Best Hits

Swiss-Prot: 59% identical to NUON_XANC5: NADH-quinone oxidoreductase subunit N (nuoN) from Xanthomonas campestris pv. vesicatoria (strain 85-10)

KEGG orthology group: K00343, NADH dehydrogenase I subunit N [EC: 1.6.5.3] (inferred from 54% identity to xfa:XF0318)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• aerobic respiration III (alternative oxidase pathway) (2/3 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• NAD(P)/NADPH interconversion (3/6 steps found)
• Fe(II) oxidation (2/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Protein Sequence (484 amino acids)

>LRK54_RS01065 NADH-quinone oxidoreductase subunit NuoN (Rhodanobacter denitrificans FW104-10B01)
MPTFNDILILLPEFYLVAAACLLLLLDAFMKPAQRPLLHWISIAVLLVAIYLVVAGQPPQ
TVTAFGGMFVRDDVAEILKVFALGTTAMVFVYARPYLIDRKLLGGEFYTLMIFAVIGIML
LVSAGNLVTVYLGLELLSLSSYALVALNRDAKLPPEAAIKYFVLGALASGMLLYGMSMVY
GASGMGGTPSLDLAQLHNVMSYTTAPTLLLFGLIFMIVGIGFKLGAAPFHMWIPDVYQGA
PTPVTTFIASGSKLAAFGMAYRLLDAGMGDLSQHWQLMLAVLAVASLAIGNLVAIVQTNL
KRMLAYSTISHMGYLLLGLSAAGPEGYAAAMFYAICYALMGVAAFGVMLAMTRAGFECEE
IADLKGLNQKSPWMAFLMLLAMFSLAGVPPLFGFWAKVLVLEAAIHANMLWLAIVGIVFA
IIGLYYYLYVVKAMYFDKPEEGGALQAQPDRPLRVVLSLNALSLLVLGLYWGPLLGWCRR
AFGL