Protein Info for LRK54_RS01025 in Rhodanobacter denitrificans FW104-10B01

Annotation: polyribonucleotide nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 701 transmembrane" amino acids 28 to 46 (19 residues), see Phobius details TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 9 to 693 (685 residues), 1071.1 bits, see alignment E=0 PF01138: RNase_PH" amino acids 14 to 143 (130 residues), 95.1 bits, see alignment E=1.2e-30 amino acids 325 to 457 (133 residues), 88.2 bits, see alignment E=1.6e-28 PF03725: RNase_PH_C" amino acids 146 to 209 (64 residues), 50.5 bits, see alignment E=4e-17 amino acids 461 to 530 (70 residues), 28.1 bits, see alignment E=4e-10 PF03726: PNPase" amino acids 241 to 321 (81 residues), 56.3 bits, see alignment E=9.5e-19 PF00013: KH_1" amino acids 557 to 615 (59 residues), 41.6 bits, see alignment 2.1e-14 PF00575: S1" amino acids 620 to 691 (72 residues), 72.2 bits, see alignment E=9.1e-24

Best Hits

Swiss-Prot: 78% identical to PNP_XANC5: Polyribonucleotide nucleotidyltransferase (pnp) from Xanthomonas campestris pv. vesicatoria (strain 85-10)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 79% identity to psu:Psesu_1825)

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (701 amino acids)

>LRK54_RS01025 polyribonucleotide nucleotidyltransferase (Rhodanobacter denitrificans FW104-10B01)
MAKVTKSFQYGNHEVTLETGEIARQASGAVMVSMGGTVVLVTAVAAAKQKEGQDFFPLTV
DYVEKFYSAGRIPGGFFKREGRPTEKETLTSRLIDRPVRPLFPEDFKNEVQVIAQVVSLN
PEVDGDIPALLGASAALSLAGIPFKGPIGAARVGYADGKYLLNPSATELKTSQLDLVVAG
TAGAVLMVESEAQLLSEDVMLGAVMFGHQQMQTAIRAIAELATEAGKPSWDWQAPARNES
LVAAVKGTVGDQLAAAFQVRDKLERRDAISAIKADVLAGLKAQAEENGWSAAAMAKEFEE
LEYHTLRDSVLKTKVRIDGRQLDDVRAITARVGVLPRTHGSALFTRGETQALVVVTLGTT
RDAQIIDAPGGESKDPFLFHYNFPPFSVGEAGRFGAPKRREIGHGRLAKRGVQAVKPSIE
AFPYVLRVVSEITESNGSSSMASVCGSSLAMMDAGVPLKAPVAGIAMGLVKEGGDFVVLS
DILGDEDHLGDMDFKVAGSAEGISALQMDIKIDGITEEIMKVALAQAKRGRLHILGEMNK
AISTARAEMSEYAPRLITIKIHPDKIREVIGKGGATIRSITEETGTTIDISDDGTIIIGS
VNRDAGETARKRIEQIVSDVEPGRIYEGKVAKLMDFGAFVTIMPGKDGLVHVSQISDERV
EKVSDKLKEGDIVKVKVLEVDKQGRIRLSMKAVTEEERAGV