Protein Info for LRK54_RS00925 in Rhodanobacter denitrificans FW104-10B01

Annotation: assimilatory sulfite reductase (NADPH) hemoprotein subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02041: sulfite reductase (NADPH) hemoprotein, beta-component
  • amino acids 6 to 550 (545 residues), 819.8 bits, E=4.7e-251
• PF03460: NIR_SIR_ferr
  • amino acids 65 to 125 (61 residues), 59 bits, E=3.1e-20
  • amino acids 343 to 408 (66 residues), 40.5 bits, E=1.8e-14
• PF01077: NIR_SIR
  • amino acids 161 to 319 (159 residues), 133.5 bits, E=4.6e-43

Best Hits

Swiss-Prot: 64% identical to CYSI_MYXXD: Sulfite reductase [NADPH] hemoprotein beta-component (cysI) from Myxococcus xanthus (strain DK 1622)

KEGG orthology group: K00381, sulfite reductase (NADPH) hemoprotein beta-component [EC: 1.8.1.2] (inferred from 68% identity to put:PT7_0173)

Predicted SEED Role

"Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (8/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.2

Use Curated BLAST to search for 1.8.1.2

Protein Sequence (565 amino acids)

>LRK54_RS00925 assimilatory sulfite reductase (NADPH) hemoprotein subunit (Rhodanobacter denitrificans FW104-10B01)
MTSHLEHIKAASRHLRGTIAEGLADPVTGAIGEDDTKLLKFHGSYQQDDRDLREERRRQK
LEPAYSFMLRARLPGGVVTPAQWLVFDRIAREHANGTLRITTRQTFQWHGIIKHRLKPTI
AAIHQALATTIAACGDVVRNVVSTANPVESAAHAEAYAWATRLSAELAPKTHAYHEIWLD
GEQLHGDTRPVEEHEPLYGATYLPRKFKIGLAIPPLNDIDVFAQDLGLIAIAEQGVLRGF
NIAVGGGMGATHGDATTYPRLASVIGFVTPEQLFGVAEAAIGVQRDWGDRTERKHARLKY
TLDRHGLDAFKAELESRLGYALAAPRAYRFEHNGDRYGWVEGHDGRWHLTLHIESGRLAD
TGERRWLTGLRELAKAHAGDFRLTCNQNVIVANVAAGERERIDAIVREHGLDGYLKQSAI
RRHAIACVALPTCGLAMAESERYLPALLPKLEARLDHHGLADAPILLRLSGCPNGCSRPY
LGEIALVGRGPGRYDLRLGADFSGQRLNQVYRENVDEAAILAALDPLFARYAAERADGEG
FGDFLRRAGILAPLPSRRIPAELVA