Protein Info for LRK54_RS00715 in Rhodanobacter denitrificans FW104-10B01

Annotation: propionate--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02316: propionate--CoA ligase
  • amino acids 1 to 625 (625 residues), 1155.2 bits, E=0
• PF16177: ACAS_N
  • amino acids 3 to 57 (55 residues), 85.2 bits, 3.2e-28
• PF00501: AMP-binding
  • amino acids 59 to 443 (385 residues), 273.1 bits, E=5.2e-85
• PF13193: AMP-binding_C
  • amino acids 510 to 589 (80 residues), 84.2 bits, E=1.5e-27

Best Hits

Swiss-Prot: 60% identical to PRPE_ECOLI: Propionate--CoA ligase (prpE) from Escherichia coli (strain K12)

KEGG orthology group: K01908, propionyl-CoA synthetase [EC: 6.2.1.17] (inferred from 78% identity to smt:Smal_0792)

MetaCyc: 60% identical to propionyl-CoA synthetase (Escherichia coli K-12 substr. MG1655)
Propionate--CoA ligase. [EC: 6.2.1.17]

Predicted SEED Role

"Propionate--CoA ligase (EC 6.2.1.17)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 6.2.1.17)

MetaCyc Pathways

• 2-methylcitrate cycle I (4/5 steps found)
• acrylate degradation II (2/3 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• 2-methylcitrate cycle II (3/6 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (5/10 steps found)
• β-alanine biosynthesis II (1/6 steps found)

KEGG Metabolic Maps

• Propanoate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.17

Protein Sequence (630 amino acids)

>LRK54_RS00715 propionate--CoA ligase (Rhodanobacter denitrificans FW104-10B01)
MRYEDFYRQSIDEPEQFWAEQARLIHWHVPPRQILDQSNPPFRRWFVGGTTNLCYNAIDR
HLAERPEQLALVAISSETGITREFTYRQLHREVNTFAAVLQSLGVGKGDRVVIYLPNIAE
AVFAMLACARIGAIHSVVFGGFAAHNLALRIDDAQPKLLVCADAGMRAGKVIPYKPLVDA
ALDEASAPPPHVLIVDRKLDPQMTRVAGRDLDYAELRAQHEDAEVPVVWLESNEPSYLLY
TSGTTGKPKGVQRDVGGYAVAMALSIRTVFDIAPGQVMFATSDVGWAVGHSYNVYGPLIG
GATSLLYEGLPTRPDAGIWWQLCERYKVRTMFSSPTAIRVLKKQDESWLKKYDLSKLKWL
FLAGEPLDEPTAQWITTALGVPVIDNYWQTETGWPAITLMPGLELKTVKFGSPGLPAPGY
RMKVIDENTGKEVPAGSKGVLVFQLPLPPGCLTTVWRDDDRYVHSYFSHFRELLYSSLDW
AVRDEDGYTFVLGRTDDVINVAGHRLGTREIEESVASHPAVAEAAVIGVKDELKGQVPIV
FATLKQDAGDGAHAVAQAMQQRVVDQLGGVAKPSRVYVVNALPKTRSGKLLRRSLQALAE
QRDPGDLSTLDDPGALDDIRRALERGPDQG