Protein Info for LRK54_RS00660 in Rhodanobacter denitrificans FW104-10B01

Annotation: glycine C-acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 399 TIGR01822: glycine C-acetyltransferase" amino acids 7 to 397 (391 residues), 724.5 bits, see alignment E=1.5e-222 PF00155: Aminotran_1_2" amino acids 43 to 386 (344 residues), 224.4 bits, see alignment E=2.7e-70 PF01212: Beta_elim_lyase" amino acids 90 to 326 (237 residues), 29.1 bits, see alignment E=6.1e-11

Best Hits

Swiss-Prot: 65% identical to KBL_ECO57: 2-amino-3-ketobutyrate coenzyme A ligase (kbl) from Escherichia coli O157:H7

KEGG orthology group: K00639, glycine C-acetyltransferase [EC: 2.3.1.29] (inferred from 82% identity to psu:Psesu_2271)

MetaCyc: 65% identical to 2-amino-3-ketobutyrate CoA ligase (Escherichia coli K-12 substr. MG1655)
Glycine C-acetyltransferase. [EC: 2.3.1.29]

Predicted SEED Role

"2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29)" in subsystem Glycine Biosynthesis or Glycine and Serine Utilization (EC 2.3.1.29)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (399 amino acids)

>LRK54_RS00660 glycine C-acetyltransferase (Rhodanobacter denitrificans FW104-10B01)
MSYSAKARYASELDAIRDQGLFKAERIIVSPQSAEIELEGGRKVLNFCANNYLGLADHPA
VIQAAKDALDSHGFGMASVRFICGTQDLHKQLEAKIAAFFGTEDTILYAACFDANGGLFE
PLLGEQDAVISDALNHASIIDGIRLCKAKRYRYANSDMADLEQQLQAADAAGARTKLIST
DGAFSMDGFIAKLDQITALAAKYDAMVHIDECHCTGFLGDSGRGSAEVNGVMDKIDIFTG
TLGKALGGALGGFTTGRKEVIELLRQRSRPYLFSNSLPPHVVAAAIKVFDMLSSAGELRE
KLKENTRYFRERMTAAGFDIKPGVHPIVPVMIYDAPKAQAMATALLEEGIYVTGFFYPVV
PQGQARIRTQMSAAHTREHLDRAIAAFTTVARKLGVIGG