Protein Info for LRK54_RS00625 in Rhodanobacter denitrificans FW104-10B01
Annotation: GTPase HflX
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 61% identical to HFLX_ECOLI: GTPase HflX (hflX) from Escherichia coli (strain K12)
KEGG orthology group: K03665, GTP-binding protein HflX (inferred from 61% identity to psu:Psesu_0805)MetaCyc: 61% identical to ribosome rescue factor HflX (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]
Predicted SEED Role
"GTP-binding protein HflX" in subsystem Hfl operon or Universal GTPases
MetaCyc Pathways
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
- pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
- UTP and CTP dephosphorylation I (5/7 steps found)
- UTP and CTP dephosphorylation II (1/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.6.1.15
Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (433 amino acids)
>LRK54_RS00625 GTPase HflX (Rhodanobacter denitrificans FW104-10B01) MFDRQKKGDRAVLVLPHSRGEGDAARRAEEFAELVKSAGAEVLGVISARVEDPNPRYYIG TGKADEVAEAARALEADLVLVDHVLTPVQERNLEKHLGIRVVDRAGLILDIFAQRARSHE GKLEVELAQLKHLATRLVRGWTHLDTQRGGAIGNRGPGETQLETDRRLLGERVKMLTKRL EKVQTQRGQQRRARLRNTVPRVALVGYTNAGKSTLFNALTTGDVYAANLLFATLDPTVRK LEGLSCGPAVLADTVGFIRELPHDLVAAFRATLAEARDADLLLHVSDAADEERDRLHHVV DKVLEEIDAGDVPQLQVMNKIDLAGAEPRIERDGTGKPVRVWLSAASGAGLDLLRQALGE LLGGERVQSALQLPLSAGRLHARLKAAGAISGETVDEHGWQLHIDAPRSVIAPLSGGDPT ETRLLRQLLAVAE