Protein Info for LRK54_RS00055 in Rhodanobacter denitrificans FW104-10B01

Annotation: 3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02737: 3HCDH_N
  • amino acids 15 to 197 (183 residues), 142.9 bits, E=2.2e-45
• PF00725: 3HCDH
  • amino acids 201 to 300 (100 residues), 45.5 bits, E=1.8e-15
• PF00378: ECH_1
  • amino acids 493 to 629 (137 residues), 55.7 bits, E=9.7e-19
• PF16113: ECH_2
  • amino acids 503 to 628 (126 residues), 40.7 bits, E=4.4e-14

Best Hits

KEGG orthology group: K07516, 3-hydroxyacyl-CoA dehydrogenase [EC: 1.1.1.35] (inferred from 74% identity to psu:Psesu_1392)

Predicted SEED Role

"Enoyl-CoA hydratase (EC 4.2.1.17) / 3,2-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Butanol Biosynthesis (EC 1.1.1.35, EC 4.2.1.17, EC 5.3.3.8)

MetaCyc Pathways

• oleate β-oxidation (32/35 steps found)
• adipate degradation (5/5 steps found)
• fatty acid β-oxidation I (generic) (6/7 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• fatty acid salvage (5/6 steps found)
• adipate biosynthesis (4/5 steps found)
• fatty acid β-oxidation III (unsaturated, odd number) (1/1 steps found)
• L-tryptophan degradation III (eukaryotic) (11/15 steps found)
• L-isoleucine degradation I (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• oleate β-oxidation (thioesterase-dependent, yeast) (1/2 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• oleate β-oxidation (reductase-dependent, yeast) (1/3 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• propanoate fermentation to 2-methylbutanoate (3/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (5/9 steps found)
• valproate β-oxidation (5/9 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (2/5 steps found)
• fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) (2/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (17/26 steps found)
• oleate β-oxidation (isomerase-dependent, yeast) (1/4 steps found)
• benzoyl-CoA degradation I (aerobic) (3/7 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• pyruvate fermentation to butanoate (3/7 steps found)
• 3-phenylpropanoate degradation (5/10 steps found)
• 6-gingerol analog biosynthesis (engineered) (2/6 steps found)
• glycerol degradation to butanol (9/16 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
• L-valine degradation I (3/8 steps found)
• pyruvate fermentation to butanol I (3/8 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (5/11 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
• phenylacetate degradation I (aerobic) (3/9 steps found)
• 2-methylpropene degradation (2/8 steps found)
• methyl tert-butyl ether degradation (3/10 steps found)
• L-glutamate degradation VII (to butanoate) (4/12 steps found)
• gallate degradation III (anaerobic) (3/11 steps found)
• superpathway of phenylethylamine degradation (3/11 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (4/13 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (6/17 steps found)
• 10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
• 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (3/16 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (3/17 steps found)
• toluene degradation VI (anaerobic) (3/18 steps found)
• androstenedione degradation I (aerobic) (8/25 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
• superpathway of testosterone and androsterone degradation (8/28 steps found)
• platensimycin biosynthesis (6/26 steps found)
• Spodoptera littoralis pheromone biosynthesis (3/22 steps found)
• androstenedione degradation II (anaerobic) (6/27 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (2/22 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (10/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (10/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (6/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Bile acid biosynthesis
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 4.2.1.17

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17 or 5.3.3.8

Protein Sequence (795 amino acids)

>LRK54_RS00055 3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase family protein (Rhodanobacter denitrificans FW104-10B01)
MTAASSPTSALRIRKAAVLGAGVMGAQIAAHLTNAGVETVLFDLPAKDGPKSGIALKAIA
NLAKLSPAPLADKNLAGAIIPANYDDDLDHLKDVDLVIEAIAERMDWKLDLYKKIAGHLS
KTAIIASNTSGLSINTLAEALPEEMRHRFSGVHFFNPPRYMHLVELIPTRLTDQNVIDGL
EAFLVTTVGKGVVIARDTPNFIGNRIGVFSMLATMHHTEQFKLGFDTVDALTGPAVGRPK
SATYRTADVVGLDTMAHVIKTMADTLPDDPWHAYFKAPVWLAGLIEKGALGQKNGAGFYR
KAGKDIVVLDVAKQDYRPSEQKPSDEVAALLAIKDPAEKFGKLRASNDPQAQFLWATFRD
LFHYTAYHLADIADTARDVDFAIRWGYGWKLGPFETWQAAGWQQVAGWIAEDIAAGKAMS
KAPLPAWVTDGRSGVHGKAGSWSASANADKPRSPHPVYRRQLFPDPILGEKFDTGSTVWE
NDGVRLWTLGDDGIGIISFKTKMHTVNDHVLDGIQHAIGIAEEKLKAVVIWQDSEPFSAG
ADLKGALGLLQAGKFDDFEKMVANFQRTSMRIKHSLVPVVAAVRGLALGGGCEFQMHSAR
TVAALESYIGLVEAGVGLLPAGGGLHELAVRAAKADPADPFEELKKVFETVAMAKVSASA
FEAKNLGLLRDSDVVVFNAYELLYVAKQVANALAESGYRPPLYARAIPVAGDVGTATFKA
SLANLQAGYFASEHDVNIATRIADTLCGGVIERGSAVDEEWLLALERKHFVELAQTEKTQ
ARIAHTMTTGKPLRN