Protein Info for IKLFDK_28425 in Pseudomonas aeruginosa PA14

Annotation: mechanosensitive channel MscK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1118 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 492 to 508 (17 residues), see Phobius details amino acids 537 to 562 (26 residues), see Phobius details amino acids 570 to 591 (22 residues), see Phobius details amino acids 616 to 633 (18 residues), see Phobius details amino acids 645 to 665 (21 residues), see Phobius details amino acids 678 to 702 (25 residues), see Phobius details amino acids 714 to 736 (23 residues), see Phobius details amino acids 780 to 803 (24 residues), see Phobius details amino acids 832 to 855 (24 residues), see Phobius details amino acids 869 to 892 (24 residues), see Phobius details amino acids 900 to 927 (28 residues), see Phobius details PF12795: MscS_porin" amino acids 37 to 273 (237 residues), 203.4 bits, see alignment E=1.1e-63 PF12794: MscS_TM" amino acids 493 to 812 (320 residues), 356.6 bits, see alignment E=2.9e-110 PF21088: MS_channel_1st" amino acids 873 to 914 (42 residues), 34.3 bits, see alignment (E = 4.6e-12) PF00924: MS_channel_2nd" amino acids 916 to 981 (66 residues), 84.3 bits, see alignment 1.2e-27 PF21082: MS_channel_3rd" amino acids 989 to 1072 (84 residues), 44.3 bits, see alignment 4.9e-15

Best Hits

KEGG orthology group: K05802, potassium efflux system protein KefA (inferred from 100% identity to pau:PA14_66400)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1118 amino acids)

>IKLFDK_28425 mechanosensitive channel MscK (Pseudomonas aeruginosa PA14)
MPALRSLIATLFLGLCLASPPLLAQSEPPSAEAVQQSLDKLAERKLAEADQKVVKARLEQ
TLKFLAARDEALQSLEDLKKRLSDAPRQIEDNQRELERLKKTRERPVSERYSGESVARLE
MLLNDRTTQQAEWQKALGEANSLSITAETRPERAQAGISSMQARILEIGSLLKAGKESGK
AINADRRGELLAEQAALTVQSQLLRQELAGNNLLQDLGKSQHDLLTEKISRLEKETLDLQ
ALISEKRREQSEKTVAELSKEGAQGAGTDSLLSQENAKNLRLSDYLLRATDRLNVLTRRN
LETKQQLDNLTQSNQALEEQINVLRGSLLLSRILYKQKQALPKIKTDQSLADEIADLRLG
QFELNQERDKLATPQQYLDDLLAQQPSEQVTPELRKDLDTLLATRSELLERLNHELNALL
NEAITLQLNQKQLLSTSESLRTTLDEQMFWIPSNQPLDLSWFKMTPTLLKNQLTEIPWGS
GVRELGEGLVDRPLLFLPLFLLIAALLWKRRYLYDKLAELNDDIGHFKRDSQLHTPLAIL
ITILLALPGTLTFAVAGLALLLDARGQNATLGSALLEMAQVWLLFYTAHRILTPGGIAER
HFHWSREQVGFLDRHLVRLGVVVLALVGVVTVAEHQPASLADDVIGIAVVLLGYAAMAWL
LSRLLFSSPGDERPSLIKMIVGLLFTALPLVLFVAVCFGYYYTSLKLTDRLIDTLYLLLL
WFVIEAAFVRGLGVAARRLAYARALAKRQNAAKEGVDGETNVEEPTLGIEQINQQSLRLI
RLALLIGFIVCLYWVWADLISVISYLDNVTLYQYTAGTGEAATSVPISLRDFLAALAIAV
VTVALARNLPGLLEVLVLQRLTLAQGSAYATTTLLSYAISGIGIVSALSTLGVSWDKLQW
LVAALSVGLGFGLQAIFSNFVSGLIILFERPVRIGDVVTIGNLSGTVSRIRIRATTITDF
DRKEIIVPNQTFITGQLVNWSLSDTVTRVTIKIGLAYETDLPLARKLMMQAVKENPRVLR
DPEPLLYFLTISASTFDYELRFHVRELGDRNPSTDEILTKIATSFREHKIDMAFNQVDVF
VKNLQGQQVQLFVGEPQHSGVGNPPALKGQDGPAEPTR