Protein Info for IKLFDK_28355 in Pseudomonas aeruginosa PA14
Annotation: glutamate synthase large subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 67% identical to GLTB_HALED: Glutamate synthase [NADPH] large chain (gltB) from Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9)
KEGG orthology group: K00265, glutamate synthase (NADPH/NADH) large chain [EC: 1.4.1.13 1.4.1.14] (inferred from 70% identity to abo:ABO_2229)MetaCyc: 61% identical to glutamate synthase subunit GltB (Escherichia coli K-12 substr. MG1655)
Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- L-glutamate and L-glutamine biosynthesis (6/7 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- superpathway of ammonia assimilation (plants) (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- ammonia assimilation cycle I (2/2 steps found)
- L-glutamate biosynthesis IV (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14
Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (1481 amino acids)
>IKLFDK_28355 glutamate synthase large subunit (Pseudomonas aeruginosa PA14) MKAGLYHPETFKDNCGFGLIAHMQGEPSHQLLQTAIEALTCMTHRGGINADGKTGDGCGL LIQKPDLFLRAVAREAFSVDLPEQYAVGMVFFNQDPVKAEAARENMNREIVAAGLELVGW RKVPIDTSVLGRLALERLPQIEQVFIGGAGLSDQDFAIKLFSARRRSSVANAADSDHYIC SFSHKTIIYKGLMMPADLAAFYPDLGDERLQTAICVFHQRFSTNTLPKWPLAQPFRFLAH NGEINTITGNRNWAQARRTKFTNELIPDLEELGPLVNRVGSDSSSMDNMLELMVTGGMDL FRGLRMIIPPAWQNVETMDADLRAFYEYNSLHMEPWDGPAGVVLTDGRYAVCLLDRNGLR PSRWVTTKNGYITLASEIGVWDYKPEDVIAKGRVGPGQILAVDTETGQVLHSDDIDNRLK SRHPYKQWLRQNALRIQATLDDDHGVASYDADQLKQFMKMFQVTFEERDQVLRPLGEQGQ EAVGSMGDDTPMAILSRRIRSPYDYFRQQFAQVTNPPIDPLREAIVMSLETCLGVEQNIF EEAPHHANQAILTTPVISPAKWRTIMTLDRPGFDRHFIDLNYDESMGLEAAVRNIADQAE EAVRGGKVLLILSDRHIAPGKLPVHAALAVGAVHHRLVQTGLRCDSNILVETATARDPHH FAVLIGFGASAVYPFLAYEVLADLIRTGEVLGDLYEVFKYYRKGISKGLMKILSKMGIST VASYRGAQLFEAVGLSDEVTGLCFTGVASRIQGARFVDIENEQKLLAAEAWSNRKPIQQG GLLKFVYGGEYHAYNPDVVNTLQAAVQQGDYEKFKEYTALVDQRPVSMIRDLLQVKTAAQ PLALDEVEPLEAIFKRFDAAGISLGALSPEAHEALAEAMNTLGGRSNSGEGGEDPARYGT LKSSKIKQVATGRFGVTPEYLVNAEVLQIKVAQGAKPGEGGQLPGGKVNGLIARLRYAVP GVTLISPPPHHDIYSIEDLAQLIFDLKQVNPQALVSVKLVSEPGVGTIAAGVAKAYADLI TISGYDGGTGASPITSIKYAGSPWELGLAETHQTLRGNDLRGKVRVQTDGGLKTGLDVIK AAILGAESFGFGTAPMIALGCKYLRICHLNNCATGVATQNDKLRKDHFIGTTAMVINFFT FIATETREWLARLGVRSLGELIGRTDLLEILPGETAKQQNLDLAPLLGSDLIPADKPQFC EVEKNPPFDQGLLAEKMVELSKAAIEGLSGGEYELDICNCDRSIGARVSGEIARLHGNQG MAKAPVVFRFKGTAGQSFGVWNAGGLHLYLEGDANDYVGKGMTGGKLVITPPKGSPFKTQ ESAIVGNTCLYGATGGKLFAAGTAGERFAVRNSGSHAVVEGTGDHCCEYMTGGFVCVLGK TGYNFGSGMTGGFAYVLDMDNTFVDRVNHELVEIQRISGEAMEAYRSHLRKVLVEYVNET ASEWGANILENLDDYLRRFWLVKPKAASLGSLLTSTRANPQ