Protein Info for IKLFDK_27690 in Pseudomonas aeruginosa PA14

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 509 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 52 to 72 (21 residues), see Phobius details amino acids 81 to 101 (21 residues), see Phobius details amino acids 109 to 131 (23 residues), see Phobius details amino acids 139 to 161 (23 residues), see Phobius details amino acids 167 to 189 (23 residues), see Phobius details amino acids 201 to 220 (20 residues), see Phobius details amino acids 232 to 251 (20 residues), see Phobius details amino acids 271 to 291 (21 residues), see Phobius details amino acids 303 to 323 (21 residues), see Phobius details amino acids 333 to 353 (21 residues), see Phobius details amino acids 364 to 386 (23 residues), see Phobius details amino acids 399 to 420 (22 residues), see Phobius details amino acids 473 to 494 (22 residues), see Phobius details PF06609: TRI12" amino acids 9 to 267 (259 residues), 26.7 bits, see alignment E=3.1e-10 TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 14 to 493 (480 residues), 515.8 bits, see alignment E=5.8e-159 PF07690: MFS_1" amino acids 19 to 413 (395 residues), 184 bits, see alignment E=5.8e-58 PF00083: Sugar_tr" amino acids 23 to 184 (162 residues), 29.7 bits, see alignment E=5e-11

Best Hits

Swiss-Prot: 50% identical to EMRB_ECO57: Multidrug export protein EmrB (emrB) from Escherichia coli O157:H7

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 99% identity to pap:PSPA7_5898)

MetaCyc: 50% identical to multidrug efflux pump membrane subunit EmrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (509 amino acids)

>IKLFDK_27690 MFS transporter (Pseudomonas aeruginosa PA14)
MSESSSFSPPSLVMATIGLSLATFMQVLDTTIANVALPTISGNLGVSSEQGTWVITSFAV
SNAIALPLTGWLARRVGEVRLFIAAALLFVLASFLCGIAQSMPSLVGFRALQGFVAGPLY
PITQTLLISIYPPAKRGMALALLAMVTVVAPIAGPILGGWITDDYSWPWIFFINVPVGLF
AAFVVYQQLKARPVVIKKAPMDYVGLIALVIGVGALQIVLDKGNDLDWFESNFIVGGALI
AAIALAFFIIWEFTDRHPIVNLRLFAHRNFAAGTLALVLGYAAFFGINLLLPQWLQTQMG
YTATWAGLAAAPIGILPVFLSPLVGRYANHFDLRILAGLSFLAMAITCFMRANFTTEVDY
QHIAIVQLIMGLGVAFFFMPILSILLSDLPPDQIADGSGLATFLRTLGGSFAASLTTWIW
NRRATQHHAYLSENISLYDPATHETLAQLGGNTQANAALLDRMVQSQAYMMSTIDYFTML
GWLFLALLVIIWLARPPFGAKPGAAASGH