Protein Info for IKLFDK_18960 in Pseudomonas aeruginosa PA14

Annotation: NAD(P)/FAD-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 620 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details transmembrane" amino acids 77 to 90 (14 residues), see Phobius details PF01266: DAO" amino acids 75 to 117 (43 residues), 28.1 bits, see alignment 2.2e-10 PF13450: NAD_binding_8" amino acids 78 to 134 (57 residues), 43.6 bits, see alignment 4.3e-15

Best Hits

KEGG orthology group: K00316, spermidine dehydrogenase [EC: 1.5.99.6] (inferred from 100% identity to pag:PLES_12691)

Predicted SEED Role

"FIG00955623: hypothetical protein"

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.99.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (620 amino acids)

>IKLFDK_18960 NAD(P)/FAD-dependent oxidoreductase (Pseudomonas aeruginosa PA14)
MTISRRDFLNGVALTIAAGLTPAEILRAAPGGRYYPPALTGLRGSHPGAFEVAHQMGWEK
KTFDVDHLPIEEEYDLVVVGGGISGLAAAWFYRERHPAARILVIENHDDFGGHAKRNEFQ
AGGRTILGYGGSESLQSPNALYSEDAKHLLKRLGVELKRFETAFDTDFYPGLGLSRAVFF
DKASFGVDKLVSGDPTPMVADEVPRDRLNARSWRAFIGDFPLSREDREALIALYESPRDY
LAGKSVEEKETYLAKTSYRDYLLKNVGLSETSVKYFQGRSNDFSALGADALPAADAYAAG
FPGFDALGLPQPSEEAQAEMDEPYIYHFPDGNASLARLMVRDLIPAVAPGRGMEDIVMAR
FDYSKLDLAGHPVRLRLNSTAVSVRNRAGGVDVGYSRAGRLHRVRGKHCVMACYNMMVPY
LLRDLSEEQAHALSQNVKFPLVYTKVLLRNWQAWKTLGIHEIYAPTLPYSRIKLDFPVDL
GSYRHPRDPRQPIGVHMVYVPTTPNAGMDARTQARVGRSKLYAMSFEQLEKDIRDQLQAM
LGPAGFDHRRDITGITVNRWSHGYSYFMNTLYDDEAESEALMELARSKVGNVTIANSDAA
WDAYAHAAIDQAVRAVRELG