Protein Info for IJEDHG_07935 in Erwinia tracheiphila HP pepo 2.2

Name: cpxA
Annotation: envelope stress sensor histidine kinase CpxA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 458 transmembrane" amino acids 7 to 28 (22 residues), see Phobius details amino acids 165 to 186 (22 residues), see Phobius details PF16527: CpxA_peri" amino acids 81 to 145 (65 residues), 42.1 bits, see alignment E=2.3e-14 PF00672: HAMP" amino acids 181 to 233 (53 residues), 45.6 bits, see alignment 1.4e-15 PF00512: HisKA" amino acids 239 to 299 (61 residues), 49.2 bits, see alignment E=8.9e-17 PF02518: HATPase_c" amino acids 346 to 454 (109 residues), 83.1 bits, see alignment E=4e-27

Best Hits

Swiss-Prot: 84% identical to CPXA_ECOLI: Sensor histidine kinase CpxA (cpxA) from Escherichia coli (strain K12)

KEGG orthology group: K07640, two-component system, OmpR family, sensor histidine kinase CpxA [EC: 2.7.13.3] (inferred from 94% identity to ebi:EbC_00900)

MetaCyc: 84% identical to sensor histidine kinase CpxA (Escherichia coli K-12 substr. MG1655)
Protein-histidine tele-kinase. [EC: 2.7.13.2]

Predicted SEED Role

"Copper sensory histidine kinase CpxA" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.2 or 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (458 amino acids)

>IJEDHG_07935 envelope stress sensor histidine kinase CpxA (Erwinia tracheiphila HP pepo 2.2)
MISSLTTRIFAIFWLTLALVLMLVLMLPKLDTRQMTPLQDNEQRQGIMIEQHIEAELIQT
PPNDLMWWRRLFRVIDKWAPPGQRLLLVTSEGRVIGAQHNEMQVIRNFIGQSDNANHPKK
KKYGRTEMLGPFSVRDGEDNYQLYLIRPAGSTQLDFINLLFDRPLLLLVVTMLISSPLLL
WLAWSLAKPARKLKHAADDVAAGNLRQRPELESGPQEFLAAGASFNQMVSALERMMTSQQ
RLLSDISHELRTPLTRLQLATALMRRRQGEGKELQRIEMEALRLDGMINDLLVLSRTQHK
NALVSEAMKASQLWSGVLADAQFEAEQMGKTLDIPYPPGPWPLYGNPSALDSALENIVRN
ALRYSYSKIMVSFSVDKRGITVHVDDDGPGVSTEDREQIFRPFYRTDEARDRESGGTGLG
LAIVETAIQQHHGWVKADDSPLGGLRLTIWLPLYTSTS