Protein Info for IAI47_20910 in Pantoea sp. MT58

Annotation: beta-galactosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 690 PF02449: Glyco_hydro_42" amino acids 19 to 392 (374 residues), 529.3 bits, see alignment E=1.1e-162 PF08532: Glyco_hydro_42M" amino acids 403 to 615 (213 residues), 252.7 bits, see alignment E=3.7e-79 PF08533: Glyco_hydro_42C" amino acids 626 to 682 (57 residues), 54.4 bits, see alignment 1.2e-18

Best Hits

Swiss-Prot: 58% identical to BGAL_ALIAD: Beta-galactosidase BglY (bglY) from Alicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / JCM 5260 / NBRC 15652 / NCIMB 11725 / NRRL B-14509 / 104-1A)

KEGG orthology group: K12308, beta-galactosidase [EC: 3.2.1.23] (inferred from 95% identity to pva:Pvag_pPag30115)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (690 amino acids)

>IAI47_20910 beta-galactosidase (Pantoea sp. MT58)
MNKFPPLSARVNRLLHGADYNPEQWAHQPGIINDDVEMMKQANCNVMSVGIFSWAKLEPE
EGRYEFGWLDEVIDTLWLQGISVFLATPSGARPAWMSQAYPEVLRTGRDRVPALHGGRHN
HCMTSPVYRQKVQAMNSRLAARYAHHPAVIGWHISNEYGGECHCDRCQEAFRGWLQRRYG
TLEALNLAWWSDFWSHTYSDWSQIVSPAPQGEMSIHGLNLDWRRFMTDQVTDFCREEIKP
LKQANPDLPATTNFMEYFYDYDYWKLAPALDFISWDSYPMWHNEKDETTLACYTAMYHDL
MRTLKQGKPFVLMESTPGATNWQPTSKLKKPGMHILSSLQAVAHGADAVQYFQWRKSRGS
VEKFHGAVIDHVGHLDTRTGREVSELGRMLAAMTPVMGSRVEARVAIIFDWESRWAMDNA
QGPRNLGLHYERTVNEHYRAFWEQGVAVDVINGDSDFSGYDLVIAPMLYMVRDGFAARAE
QHVERGGHFVASYWSGIVNESDLCYPGGFPGPLRPLLGIWSEEIDSLTDEEFNGVRGVRG
NELGLSGPYQARELCEHIHLEGATALASYESDFYAGTPAVTMNRVGQGKAWYIASRNDLS
FHRDFYGALIKQLGLPRALAGELPPGVVVQRRTDGEQAFLFVQNFTAQTQQLSLPAGLSD
LVDGMSVGGSLTLGPWGCRVLSGPMAQGAV