Protein Info for IAI47_20570 in Pantoea sp. MT58

Annotation: glycine betaine/L-proline transporter ProP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 488 transmembrane" amino acids 51 to 69 (19 residues), see Phobius details amino acids 75 to 99 (25 residues), see Phobius details amino acids 111 to 131 (21 residues), see Phobius details amino acids 137 to 159 (23 residues), see Phobius details amino acids 179 to 200 (22 residues), see Phobius details amino acids 211 to 232 (22 residues), see Phobius details amino acids 270 to 288 (19 residues), see Phobius details amino acids 305 to 324 (20 residues), see Phobius details amino acids 336 to 355 (20 residues), see Phobius details amino acids 361 to 383 (23 residues), see Phobius details amino acids 397 to 419 (23 residues), see Phobius details amino acids 431 to 448 (18 residues), see Phobius details PF00083: Sugar_tr" amino acids 43 to 251 (209 residues), 126.5 bits, see alignment E=1.4e-40 amino acids 252 to 457 (206 residues), 54.6 bits, see alignment E=9e-19 PF07690: MFS_1" amino acids 84 to 407 (324 residues), 122.2 bits, see alignment E=2.5e-39

Best Hits

Swiss-Prot: 52% identical to PROP_ECO57: Proline/betaine transporter (proP) from Escherichia coli O157:H7

KEGG orthology group: K03762, MFS transporter, MHS family, proline/betaine transporter (inferred from 97% identity to pva:Pvag_pPag30193)

MetaCyc: 52% identical to osmolyte:H+ symporter ProP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-29; TRANS-RXN-29A

Predicted SEED Role

"L-Proline/Glycine betaine transporter ProP" in subsystem Proline, 4-hydroxyproline uptake and utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (488 amino acids)

>IAI47_20570 glycine betaine/L-proline transporter ProP (Pantoea sp. MT58)
MDTRNSSSEHPKKHFWNRKPKELTVDDITVIDNDMLKRAVGAAALGNAMEWFDFGVYSYL
AVIIGKVFFPDANNAVQLIATFGTFAAAFLVRPIGGLVFGPLGDRIGRQKVLAMTMIMMS
IGTFCIGLIPAYSSIGIMAPILLLVARLIQGFSTGGEYGGAATFIAEYSTDKRRGFMGSF
LEFGTIGGYLMGASLVTVMTTVMSNEAMMSWGWRVPFFIAAPLGLFGLYVRLKLEETPAF
QQHMEKQEALEQSKPRLTLMQMLSKYRAPMMKCIGLVLLFNVSNYMLTSYMPSYLTGILG
LPELSGLLLVMVAMFVMMPLTLLWGRWTDRIGRRPVIAFGAVGLIILAIPSFMLIGSGNM
WAVFGGLMILGVLHTCFSGTMPSTLPALFTTDIRYSALAIGFNLSVSLFGGTTPLITAWL
VDTTKNTMMPAYYMMGAGIIGLLTILTVRETARQPLTGSSPAVATKAEAHRLIDKLRKRN
KPAATAGK