Protein Info for IAI47_20425 in Pantoea sp. MT58

Annotation: flavocytochrome c

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 926 transmembrane" amino acids 905 to 924 (20 residues), see Phobius details PF00724: Oxidored_FMN" amino acids 7 to 338 (332 residues), 249.7 bits, see alignment E=2.3e-77 PF04205: FMN_bind" amino acids 395 to 461 (67 residues), 25.4 bits, see alignment 8.5e-09 PF03486: HI0933_like" amino acids 482 to 672 (191 residues), 37.3 bits, see alignment E=6.4e-13 PF07992: Pyr_redox_2" amino acids 482 to 671 (190 residues), 47.5 bits, see alignment E=7.5e-16 PF00890: FAD_binding_2" amino acids 483 to 906 (424 residues), 265.5 bits, see alignment E=4.7e-82 PF01134: GIDA" amino acids 483 to 675 (193 residues), 33 bits, see alignment E=1.7e-11 PF01266: DAO" amino acids 483 to 584 (102 residues), 30.1 bits, see alignment E=1.8e-10 TIGR01813: flavocytochrome c" amino acids 483 to 917 (435 residues), 500.2 bits, see alignment E=2.4e-154 PF12831: FAD_oxidored" amino acids 483 to 668 (186 residues), 48.6 bits, see alignment E=3.7e-16 PF13450: NAD_binding_8" amino acids 486 to 543 (58 residues), 30.9 bits, see alignment 1.2e-10

Best Hits

KEGG orthology group: K00244, fumarate reductase flavoprotein subunit [EC: 1.3.99.1] (inferred from 98% identity to pva:Pvag_pPag30231)

MetaCyc: 73% identical to fumarate reductase (NADH) (Klebsiella pneumoniae)
Fumarate reductase (NADH). [EC: 1.3.1.6]

Predicted SEED Role

"Fumarate reductase flavoprotein subunit (EC 1.3.99.1)" in subsystem Succinate dehydrogenase (EC 1.3.99.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.1

Use Curated BLAST to search for 1.3.1.6 or 1.3.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (926 amino acids)

>IAI47_20425 flavocytochrome c (Pantoea sp. MT58)
MKSLTPLLNPLTLPNGAVLKNRLVMAPMTTCTGFYDGTVTSELVEYYRARAGSIGTVIVE
CCFIDNRGPAFPGAIAIDSDNKIPGLKRIADAIKSEGSTAILQIYHGGRMVEPELIGGKT
PVAPSAIAAPREGATQPLALSAEEVDTMITKFGDAVNRAIKAGFDGVEIHGANTYLIQQF
YSPNSNQRDDKWGGSRDNRARFPLEVLEITHKMAERFAHNSFIIGYRFSPEEIEVPGIRF
DDTLYLLEKLAARGLDYVHFSVGQLLRPSIVDTQDPTPLIEKFVAMRSPELAKVPVIGVG
GVVNKADAELALEKGYDLVAIGKACIAYPNWTDRIINNEHLELYIDSNKREELQIPEPLW
RFSLVDAMIRDTSESGRKYKAGVYQEKVEAEALKLKINVTLDTDRITDISLVPDDTLDVD
FTTTFESLRSRILVANSPHVDAITGATTQSEALKKAVSRAMTTSSKEHVIEEGGNPNAPQ
NYDVVIVGSGGAGLAAAIQAHDEGARVVIIEKMPTIGGNTIKASVGMNAAETRFQKLKGI
EDSKELFYEETLKGGQFKNNPVLLREFVDLAPEAIEWLADKGIELNDITITGGMSIDRTH
RPADRSAVGGFLISGLVKNINSRNIEVLLETSVAEILYENGAVAGVKVVDEYNDSRILNA
KSVIVATGGFSANRELVVKYRPELDGFVTTNHKGATGSGIAMLEKIGAGTVDMGEIQIHP
TVEQTTSYLVSESIRGGGAILVSQAGERFYNEMETRDKVSAQIIALPEKSAWIVFDEQVR
MNNKAADEYIAKGFVISAPTVAELAVKLNMSEHHTLEATLARYNNFVIMQKDEDFGRQTA
LRHPLSEAPFYAIRVAPGVHHTMGGVTIDTTTAVLDSQKQVINGAWACGEVVGGIHGANR
IGGNAVADIIIFGILAGGNAAAYARR