Protein Info for IAI47_20405 in Pantoea sp. MT58

Annotation: malate dehydrogenase (quinone)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06039: Mqo
  • amino acids 30 to 516 (487 residues), 768.3 bits, E=2.9e-235
• TIGR01320: malate dehydrogenase (acceptor)
  • amino acids 31 to 514 (484 residues), 670.7 bits, E=6.1e-206
• PF01266: DAO
  • amino acids 32 to 303 (272 residues), 38 bits, E=1.5e-13

Best Hits

Swiss-Prot: 81% identical to MQO_CROS8: Probable malate:quinone oxidoreductase (mqo) from Cronobacter sakazakii (strain ATCC BAA-894)

KEGG orthology group: K00116, malate dehydrogenase (quinone) [EC: 1.1.5.4] (inferred from 98% identity to pva:Pvag_pPag30235)

MetaCyc: 69% identical to malate:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
MALATE-DEHYDROGENASE-ACCEPTOR-RXN [EC: 1.1.5.4]

Predicted SEED Role

"Malate:quinone oxidoreductase (EC 1.1.5.4)" in subsystem TCA Cycle (EC 1.1.5.4)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.5.4

Use Curated BLAST to search for 1.1.5.4

Protein Sequence (549 amino acids)

>IAI47_20405 malate dehydrogenase (quinone) (Pantoea sp. MT58)
MMNSKKVIPTLSLAALLVSSALHAETSPEKTDVLLIGGGIMSASMGTMLEELQPGWKQIM
VEKLDGVALESSNGWNNAGTGHSANMELNYTPERKDGSIDVSKALEINEAFMISRQFWSS
QVKEGVMHDPRSFINSTPHMSFVWGDNVDFLTKRYAALQKTALFQGMKFSTDHKQIEQWA
PLVMEGRDPQQKVAATWTPVGTDVNYGEITRQLIGSLKKNDNFRLETSSEVTDFKRNGDN
SWHVTIKDAKNGNERAVDAKYVFIGAGGGALKLLQKTGIPEADNYAGFPVGGSFLVTENK
AIADRHLAKVYGQASTGAPPMSVPHLDTRYLDGKRVVLFGPFATFSTKFLKNGSLFDLLS
TTTTHNIVPMTDVGMDNFDLVKYLIGQVMLSDEDRFKALQEYYPDAKKEDWKLIQAGQRV
QIIKKDADKGGVLKLGTEIVTDQQKTVAALLGASPGASTAAPISLSVMQKLFPEEFKSPE
WQAKIHKMIPGYGQKLNDNVPLLQQVWNETAQTLQLTTPPVINMEGKTTDAPAAQPEKAA
PSAKHDMAL