Protein Info for IAI47_19435 in Pantoea sp. MT58

Annotation: 23S rRNA pseudouridine(2604) synthase RluF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 292 PF01479: S4" amino acids 8 to 52 (45 residues), 38.4 bits, see alignment 8.3e-14 PF00849: PseudoU_synth_2" amino acids 69 to 195 (127 residues), 71.9 bits, see alignment E=7.1e-24 TIGR00093: pseudouridine synthase" amino acids 73 to 226 (154 residues), 174 bits, see alignment E=1e-55

Best Hits

Swiss-Prot: 81% identical to RLUF_SALTI: 23S rRNA pseudouridine(2604) synthase (rluF) from Salmonella typhi

KEGG orthology group: K06182, ribosomal large subunit pseudouridine synthase F [EC: 5.4.99.12] (inferred from 98% identity to pva:Pvag_pPag30466)

MetaCyc: 80% identical to 23S rRNA pseudouridine2604 and tRNATyr pseudouridine35 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11835 [EC: 5.4.99.21]; 5.4.99.- [EC: 5.4.99.21]

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase F (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12 or 5.4.99.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (292 amino acids)

>IAI47_19435 23S rRNA pseudouridine(2604) synthase RluF (Pantoea sp. MT58)
MLTDSSTRLNKYISESGICSRRDADRYIEQGNVFINGKRASVGAQVFAGDVVKVNGQLIE
PRDEDDLVLIALNKPVGIITTMEEGERDNISDFVNHSKRVFPIGRLDKDSQGLIFLTNHG
DLVNKILRAGNNHEKEYVVTVDKPITDEFIAGMSAGVPMLGTVTKKCKVKKEAPFVFRIT
LVQGLNRQIRRMCKHFGFEVTKLERTRIMNVNLKGLPLGEWRDLTDDELVELFKLLENSS
SEDKPAKKAKAPAKAAAASKPQKRAAKPAEKADANGASRKRFVQPGRKKKGR