Protein Info for IAI47_18785 in Pantoea sp. MT58

Annotation: glycine--tRNA ligase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 689 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 1 to 689 (689 residues), 1049.2 bits, see alignment E=0 PF02092: tRNA_synt_2f" amino acids 6 to 551 (546 residues), 719.8 bits, see alignment E=2.6e-220 PF05746: DALR_1" amino acids 581 to 679 (99 residues), 40.5 bits, see alignment E=2.8e-14

Best Hits

Swiss-Prot: 87% identical to SYGB_ERWT9: Glycine--tRNA ligase beta subunit (glyS) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 87% identity to ebi:EbC_45080)

MetaCyc: 83% identical to glycine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glycine--tRNA ligase. [EC: 6.1.1.14]

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (689 amino acids)

>IAI47_18785 glycine--tRNA ligase subunit beta (Pantoea sp. MT58)
MTEQTFLVEIGTEELPPKALRSLAEAFAAQVTAELDAANLSHGEVSWFAAPRRLALKVAN
LSASQPDREVEKRGPAVSAAFDADGNATKAAEGWARGNGITVDQAERLATDKGEWLVYRA
QVKGEAAQALLPAMIATALSKLPVPKLMRWGDSDVQFVRPVHTVTMLLGDELIPGTILGI
KSDRVIRGHRFMGESEITLDNADQYPDVLEKRGQVIADYQVRKAMIKADAEAAARRLGGN
ADISDSLLEEVTSLVEWPVVLTATFEEKFLKVPAEALVYTMKGDQKYFPVYDNDGNLMPN
FIFVTNIESSEPRQIISGNEKVVRPRLADAEFFFNTDRKKRLEDHLPRLETVLFQKELGT
LRDKTDRIQALASWIAGEIGADVNHATRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
GEAEDVAVALNEQYQPRFAGDDLPSSPVACALAIADKMDTLAGIFGIGQHPKGDKDPFAL
RRAALGVLRIIVEKNLPLDLQTLTEEAVRLYGSKLSNARVVDDVIDFMLGRFRTWYQEEG
HSIDTLQAVLARRPTRPADFDARMKAVSHFRTLEAASALAAANKRVSNILAKSTETLNES
VQASLLKENEEIQLATFVTALSSKLQPYFAEGRYQDALVELAQLRTAVDNFFDKVMVNAD
DQAVRVNRLTLLSKLRELFLQVADISLLQ