Protein Info for IAI47_18425 in Pantoea sp. MT58

Annotation: hydroxymethylbilane synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01379: Porphobil_deam
  • amino acids 6 to 212 (207 residues), 285.6 bits, E=2.1e-89
• TIGR00212: hydroxymethylbilane synthase
  • amino acids 7 to 296 (290 residues), 373.4 bits, E=3.3e-116
• PF03900: Porphobil_deamC
  • amino acids 227 to 296 (70 residues), 79.8 bits, E=1.6e-26

Best Hits

Swiss-Prot: 82% identical to HEM3_ERWT9: Porphobilinogen deaminase (hemC) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K01749, hydroxymethylbilane synthase [EC: 2.5.1.61] (inferred from 98% identity to pva:Pvag_3401)

MetaCyc: 77% identical to hydroxymethylbilane synthase (Escherichia coli K-12 substr. MG1655)
Hydroxymethylbilane synthase. [EC: 2.5.1.61]

Predicted SEED Role

"Porphobilinogen deaminase (EC 2.5.1.61)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 2.5.1.61)

MetaCyc Pathways

• superpathway of heme b biosynthesis from glutamate (10/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
• superpathway of heme b biosynthesis from glycine (7/8 steps found)
• dipyrromethane cofactor biosynthesis (3/4 steps found)
• tetrapyrrole biosynthesis II (from glycine) (3/4 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (13/33 steps found)
• superpathway of bacteriochlorophyll a biosynthesis (6/26 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (12/36 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.61

Protein Sequence (313 amino acids)

>IAI47_18425 hydroxymethylbilane synthase (Pantoea sp. MT58)
MLDKIFRIATRQSPLALWQAHYVQQRLMSAHPGLRVELVPMVTKGDIILDTPLAKVGGKG
LFVKELELAMQDGRADMAVHSMKDVPVSFPEGLGLVTICERDDPRDAFVSHHYDSIDALP
QGAIVGTSSLRRQCQISARRPDLVIRSLRGNVGTRLGKLDAGEYDAIILAVAGLKRLGLE
DRIRQAMPAEVSLPAVGQGAVGIECRLDDSVLIDLLRALNHDDTEVCVRAERAMNTRLEG
GCQVPIGSFAVLEGNDLWLRGMVGAPDGRVMVSGERRGPRAQAEQMGISLADELLNGGAR
EILKEVYQGQPPA