Protein Info for IAI47_18285 in Pantoea sp. MT58

Annotation: bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 252 PF01209: Ubie_methyltran" amino acids 21 to 251 (231 residues), 348.7 bits, see alignment E=4.9e-108 TIGR01934: ubiquinone/menaquinone biosynthesis methyltransferase" amino acids 26 to 251 (226 residues), 287.3 bits, see alignment E=3.3e-90 PF01135: PCMT" amino acids 62 to 169 (108 residues), 31.9 bits, see alignment E=4.2e-11 PF13489: Methyltransf_23" amino acids 63 to 235 (173 residues), 52.9 bits, see alignment E=1.3e-17 PF13847: Methyltransf_31" amino acids 64 to 180 (117 residues), 74.9 bits, see alignment E=2.1e-24 PF13649: Methyltransf_25" amino acids 68 to 165 (98 residues), 83.3 bits, see alignment E=5.7e-27 PF08241: Methyltransf_11" amino acids 69 to 169 (101 residues), 79.9 bits, see alignment E=6.4e-26 PF08242: Methyltransf_12" amino acids 69 to 167 (99 residues), 46.7 bits, see alignment E=1.6e-15

Best Hits

Swiss-Prot: 88% identical to UBIE_ERWT9: Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE (ubiE) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K03183, ubiquinone/menaquinone biosynthesis methyltransferase [EC: 2.1.1.- 2.1.1.163] (inferred from 100% identity to pva:Pvag_3430)

MetaCyc: 84% identical to bifunctional 2-octaprenyl-6-methoxy-1,4-benzoquinol methylase and demethylmenaquinone methyltransferase (Escherichia coli K-12 substr. MG1655)
2-OCTAPRENYL-METHOXY-BENZOQ-METH-RXN [EC: 2.1.1.201]; ADOMET-DMK-METHYLTRANSFER-RXN [EC: 2.1.1.201, 2.1.1.163]

Predicted SEED Role

"Ubiquinone/menaquinone biosynthesis methyltransferase UbiE (EC 2.1.1.-)" in subsystem Menaquinone Biosynthesis via Futalosine or Menaquinone and Phylloquinone Biosynthesis or Ubiquinone Biosynthesis (EC 2.1.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.163 or 2.1.1.201

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (252 amino acids)

>IAI47_18285 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE (Pantoea sp. MT58)
MADESQQETTHFGFQTVAKSEKADKVADVFHSVAAKYDLMNDLMSFGVHRIWKRFTIDSS
GVRRGQRVLDLAGGTGDLTAKFSRLVGETGQVVLADINSSMLKMGREKLRNQGVVGNVSY
VQANAEALPFPDNYFDCITISFGLRNVTEKEKALASMFRVLKPGGRLLVLEFSKPVLDPL
SKAYDAYSFHILPRIGQLVAQDAESYRYLAESIRMHPDQETLKAMMNDVGFENTTYSNMT
GGIVALHRGFKF