Protein Info for IAI47_18000 in Pantoea sp. MT58

Annotation: bifunctional isocitrate dehydrogenase kinase/phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 582 transmembrane" amino acids 252 to 270 (19 residues), see Phobius details amino acids 316 to 332 (17 residues), see Phobius details PF20423: AceK_regulatory" amino acids 9 to 305 (297 residues), 346.9 bits, see alignment E=9.6e-108 PF06315: AceK_kinase" amino acids 308 to 561 (254 residues), 394.2 bits, see alignment E=2.8e-122

Best Hits

Swiss-Prot: 66% identical to ACEK_CROS8: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Cronobacter sakazakii (strain ATCC BAA-894)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 93% identity to pva:Pvag_3490)

MetaCyc: 65% identical to isocitrate dehydrogenase kinase/phosphatase (Escherichia coli K-12 substr. MG1655)
3.1.3.-; [Isocitrate dehydrogenase (NADP(+))] kinase. [EC: 2.7.11.5]

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (582 amino acids)

>IAI47_18000 bifunctional isocitrate dehydrogenase kinase/phosphatase (Pantoea sp. MT58)
MPPRESLIAHTILQGFDAQYGRFLDITAGAQQRFEQAEWQAVQHAMKARIHLYDHHVSLV
ADQLRVLNGAASWDAMFWLRVKDHYQSLLPGYPRHEIAESFFNSVYCRLHGHADLQPDRL
FIFSSQSLTVPVTPLRPLSRRYQPERGWRALVDQILEDLPLTLPWQHRARDVGWIVRHLE
SHFPRLSEAWLEVNSELFYRNKTAWLVARLHLPAGIFPCLLPIQRTETGELWIDTCLTDV
NDASIVFGFARAYFMVYAPVPAALVAWLQPILPVKTLAERYMAIGCQKHGKTESYREYLQ
ALAESETAFEIAPGVRGMVMLVFTLPGFDWVFKVIRDRFAPQKEVTEAQVRACYQQVKEH
DRVGRMADTQEFEQFALPLARISPALLAELHACVAEKLYIEGDRLIIRHLWLERRMQPLN
LYLAQASPAERRHAIEEYGNAIRQLAAANIFPGDMLFKNFGITRHGRVIFYDYDEIRPMQ
ELNFREVPAARYEEDELSAEPWYSVGPDDVFPETFRYALCSETATGALLLELHPEIFDAS
WWRAQQQRIAQGHVEEVIAWRQTQRFSVCYTPERVSDRCTPA