Protein Info for IAI47_17085 in Pantoea sp. MT58

Annotation: serine-type D-Ala-D-Ala carboxypeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 477 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF02113: Peptidase_S13" amino acids 21 to 468 (448 residues), 529.6 bits, see alignment E=6.4e-163 TIGR00666: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase" amino acids 46 to 447 (402 residues), 531.1 bits, see alignment E=7.3e-164 PF13354: Beta-lactamase2" amino acids 285 to 442 (158 residues), 22.5 bits, see alignment E=7e-09

Best Hits

Swiss-Prot: 74% identical to DACB_ECOLI: D-alanyl-D-alanine carboxypeptidase DacB (dacB) from Escherichia coli (strain K12)

KEGG orthology group: K07259, D-alanyl-D-alanine carboxypeptidase / D-alanyl-D-alanine-endopeptidase (penicillin-binding protein 4) [EC: 3.4.16.4 3.4.21.-] (inferred from 100% identity to pva:Pvag_3667)

MetaCyc: 74% identical to peptidoglycan DD-endopeptidase DacB (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]; 3.4.-.- [EC: 3.4.16.4]

Predicted SEED Role

"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4, 3.4.21.-

Use Curated BLAST to search for 3.4.16.4 or 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (477 amino acids)

>IAI47_17085 serine-type D-Ala-D-Ala carboxypeptidase (Pantoea sp. MT58)
MRFSRLVTGLSCAFMLQAHAAPVDEYMQYLPDGANLALMVQKVGASTPIIDYHGKQMALP
ASTMKVVTALAALLELGGDFRFQTTFETRGAIIDGTLNGDLVARFGGDPTLTRQDLRNMV
AAIKKQGINHIKGNLVIDTSVFASHDMAPGWPWNDMTQCFSAPPGAAIVDKNCFSVSLYS
ANTPGENAFVRIASYYPAHMYSQVRTIGRNSGEGQYCELDVVPGELNRYTLTGCMRQRAE
PLPLAFAVQDGAAWAGEILKAELRSSEIDYSGHLVRQTQVTEPGTVLASTQSAPLHTLLH
TMLKKSDNMIADTVFRTIGHHYFNVPGTFRAGQDAVRRILKAKANVDMGNSIQVDGSGLS
RHDLISPQTMMQVLQFIAKNDNTLDYISMLPLAGYDGTLQYRGGLHEAGLDGKVSAKTGS
LQGVYNLAGFITTSSGARVAFVQFLSGYAVPPEDQRTRRIPLVRFESRLYRDINQNN