Protein Info for IAI47_16880 in Pantoea sp. MT58

Annotation: bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 716 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details transmembrane" amino acids 42 to 61 (20 residues), see Phobius details amino acids 415 to 438 (24 residues), see Phobius details PF01553: Acyltransferase" amino acids 26 to 137 (112 residues), 66.2 bits, see alignment E=2.8e-22 PF00501: AMP-binding" amino acids 233 to 568 (336 residues), 193.4 bits, see alignment E=5.8e-61

Best Hits

Swiss-Prot: 82% identical to AAS_ERWT9: Bifunctional protein Aas (aas) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K05939, acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase / long-chain-fatty-acid--[acyl-carrier-protein] ligase [EC: 2.3.1.40 6.2.1.20] (inferred from 98% identity to pva:Pvag_3708)

MetaCyc: 72% identical to fused 2-acylglycerophospho-ethanolamine acyltransferase/acyl-acyl carrier protein synthetase (Escherichia coli K-12 substr. MG1655)
RXN-5741 [EC: 6.2.1.47]; 6.2.1.- [EC: 6.2.1.47]; Acyl-[acyl-carrier-protein]--phospholipid O-acyltransferase. [EC: 6.2.1.47, 2.3.1.40]

Predicted SEED Role

"2-acylglycerophosphoethanolamine acyltransferase (EC 2.3.1.40) / Acyl-[acyl-carrier-protein] synthetase (EC 6.2.1.20)" (EC 2.3.1.40, EC 6.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.40 or 6.2.1.20 or 6.2.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (716 amino acids)

>IAI47_16880 bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase (Pantoea sp. MT58)
MAFTFLRLIFRLLWRVELRGDVAELHKQKVLITPNHMSFLDGMLLALFLPVKPVFAVYST
ISESWFMRLVKPFIDFVPLDPTRPMALKQLVRLIDTGRPVVIFPEGRITVTGSLMKIYSG
AGFVAARSGANVVPMRIEGAEYTPFGRLAGVFKRRLFPRITLTVLPATRIPMPEAKRARD
RRVLAGEHLHHVMMEARMAVRPRETLFEAFLAARTRYGAFKRCIEDVNFKPDSYNGLLKK
SLGVGRILERYSQPREVVGLLLPNTTVTAAAIFGATLRGRVPAMLNYSAGVNGMRAALTA
AQIKTVFTSRQFLDKGNLWHLPQGLDDVQWIYLEDLKDTVTGKDKLWILAHLLMPHRAQL
PQQPEDAAMVLFTSGSEGNPKGVVHSHKSLLANVEQIRTIADFTPRDRFMSALPLFHAFG
LTVGLFTPLMTGAQVFLYPSPLHYRIVPELVYDRNCTVLFGTSTFLGNYARFAAPYDFAR
LRYVVAGAEKLQENTRQLYIEKYGIRILEGYGVTECAPVVAINVPMAAKTHTVGRILPGM
DSRLMSVPGIEQGGRLQLRGPNVMKGYLRVENPGVLEAPAADDGEGNLEAGWYDTGDIVS
FDEGGFCQIQGRAKRFAKIAGEMVSLEIVEQIALKASPEKQHAASLRPDGQRGEALVLFT
TDAELVRDRLQKAARELGAPELALPRDIRVMKQLPQLGSGKPDYVTLKKMAEEEQA