Protein Info for IAI47_16805 in Pantoea sp. MT58

Annotation: valine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 951 transmembrane" amino acids 503 to 524 (22 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 1 to 930 (930 residues), 1266.9 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 631 (618 residues), 544.3 bits, see alignment E=4.5e-167 PF09334: tRNA-synt_1g" amino acids 41 to 106 (66 residues), 31 bits, see alignment 3e-11 PF08264: Anticodon_1" amino acids 674 to 825 (152 residues), 157 bits, see alignment E=8e-50 PF10458: Val_tRNA-synt_C" amino acids 887 to 944 (58 residues), 59 bits, see alignment 1.1e-19

Best Hits

Swiss-Prot: 87% identical to SYV_ECO57: Valine--tRNA ligase (valS) from Escherichia coli O157:H7

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 76% identity to aha:AHA_3702)

MetaCyc: 87% identical to valine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Valine--tRNA ligase. [EC: 6.1.1.9]; 6.1.1.9 [EC: 6.1.1.9]; 3.1.1.- [EC: 6.1.1.9]; RXN-23924 [EC: 6.1.1.9]

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (951 amino acids)

>IAI47_16805 valine--tRNA ligase (Pantoea sp. MT58)
MEKTYNPQDIEQPLYEHWEKQGYFKPNGDTSQESFCIMIPPPNVTGSLHMGHAFQQTIMD
TMIRYQRMQGKNTLWQAGTDHAGIATQMVVERKIAAEEGKTRQDYGRDAFIDKIWQWKAE
SGGTITRQMRRLGNSVDWERERFTMDDGLSNAVKEVFVRLYKENLIYRGKRLVNWDPKLR
TAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLVVATTRPETLLGDTGVAVNPEDP
RYKDLIGKFVMLPLVGRLIPIVGDEHADMEKGTGCVKITPAHDFNDYEVGRRHKLPMINI
LTFDGDIRDRAQVYDTNGEASDACSDEIPAAFQHMERFAARKAIVAAVDELGLLEEIKPH
DLTVPYGDRGGVVIEPMLTDQWYVRTAPLAKVAVEAVENGDIQFVPKQYENMYFSWMRDI
QDWCISRQLWWGHRIPAWYDDNGNVYVGRTEEEVRAENNLAADVALRQDDDVLDTWFSSG
LWTFSTLGWPENTEALRTFHPTSVLVSGFDIIFFWIARMIMLTMHFIKDENGKPQVPFKT
VYMTGLIRDEEGQKMSKSKGNVIDPLDMVDGISLEALLEKRTGNMMQPQLAEKIRKRTEK
QFPDGIEPSGTDALRFTLAALASTGRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEDQD
CGQNGGDMTLSLADRWILTEFNNTVKAYREALDSYRFDIAANLLYDFTWNQFCDWYLELT
KPVMNSGSESELRGTRHTLVHVLEALLRLAHPIIPFITETIWQRVKGLKNISDDTIMLQP
FPHYDASKEDAEAKADIEWMKQAIVAVRNIRAEMNIALSKPLDVLLRDCSPAALRRVEAN
RNFLSRLARLESLTILPAGEKGPVSVTKLVEGAELLIPMADLVDKTAELERLAKELVKLD
VEIEKIETKLANEGFVSRAPEAVVAKERERVADLQQSKAKLIEQQAVIAAL