Protein Info for IAI47_16215 in Pantoea sp. MT58

Annotation: bifunctional aspartate kinase/homoserine dehydrogenase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 820 PF00696: AA_kinase" amino acids 2 to 284 (283 residues), 155.1 bits, see alignment E=6.5e-49 TIGR00657: aspartate kinase" amino acids 3 to 460 (458 residues), 488.7 bits, see alignment E=9.3e-151 PF13840: ACT_7" amino acids 314 to 376 (63 residues), 35.7 bits, see alignment 1.6e-12 amino acids 393 to 456 (64 residues), 38.2 bits, see alignment 2.6e-13 PF01842: ACT" amino acids 405 to 448 (44 residues), 27 bits, see alignment 7.7e-10 PF03447: NAD_binding_3" amino acids 472 to 605 (134 residues), 92.5 bits, see alignment E=7.8e-30 PF00742: Homoserine_dh" amino acids 614 to 811 (198 residues), 163.9 bits, see alignment E=8.4e-52

Best Hits

Swiss-Prot: 84% identical to AK1H_ECOLI: Bifunctional aspartokinase/homoserine dehydrogenase 1 (thrA) from Escherichia coli (strain K12)

KEGG orthology group: K12524, bifunctional aspartokinase / homoserine dehydrogenase 1 [EC: 1.1.1.3 2.7.2.4] (inferred from 90% identity to ebi:EbC_06600)

MetaCyc: 84% identical to fused aspartate kinase/homoserine dehydrogenase 1 (Escherichia coli K-12 substr. MG1655)
Aspartate kinase. [EC: 2.7.2.4]; Homoserine dehydrogenase. [EC: 2.7.2.4, 1.1.1.3]

Predicted SEED Role

"Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis or Methionine Biosynthesis (EC 1.1.1.3, EC 2.7.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.3, 2.7.2.4

Use Curated BLAST to search for 1.1.1.3 or 2.7.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (820 amino acids)

>IAI47_16215 bifunctional aspartate kinase/homoserine dehydrogenase I (Pantoea sp. MT58)
MRVLKFGGTSVANAERFLRVADILESNAQQGQVATVLSAPAKITNHLVAMIEKTISGQDA
LPNISDAERIFSELLQGLADAQPGFEFDRLKTRVDLEFAQLKQVLHGISLLGQCPDSVNA
AIICRGEKLSIAIMEALLQARGHEVSVIDPVEKLLAIGHYLESTVDIAESTRRIEASQIP
PQNMILMAGFTAGNERGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV
PDARLLKSMSYQEAMELSYFGAKVLHPRTIAPIAQFQIPCLIKNTANPQAPGTLIGAEGE
PDESPVKGITNLNNMAMVNVSGPGMKGMVGMAARVFAAMSRTGISVVLITQSSSEYSISF
CVPQSELARARHVLEEEFYLELKDGLLDPLDIIENLAVISVVGDGMRTLRGISAKFFSAL
ARANINIVAIAQGSSERSISVVVNNDEVITGVRVVHQMLFATDQVIEVFVVGVGGVGGAL
LDQLHRQQAWLKKKHIDLRVCGIANSQALLTHVHGIDLSNWQSALAEAKEPFNLGRLTRL
VKEYHLLNPVIVDCTSSQAVADQYADFLSEGFHVVTPNKKANTSSWNYYQQMRAAAAKSR
RKFLYDTNVGAGLPVIENLQNLMNAGDELLRFSGILSGSLSFIFGKLDEGVSLSQATNMA
REMGFTEPDPRDDLAGTDVARKLLILAREAGHQLEMSDIEIEPLLPASLTEIQDVEQFMQ
RLPELDNAFADRVAQAQNEGKVLRYVGVIEEGGVCKVKVDAVDGNDPLYKVKNGENALAF
YSRYYQPIPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV