Protein Info for IAI47_16150 in Pantoea sp. MT58

Annotation: bifunctional riboflavin kinase/FAD synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06574: FAD_syn
  • amino acids 12 to 167 (156 residues), 194.5 bits, E=1.6e-61
• TIGR00083: riboflavin biosynthesis protein RibF
  • amino acids 18 to 306 (289 residues), 380 bits, E=7.9e-118
• PF01467: CTP_transf_like
  • amino acids 19 to 170 (152 residues), 24.7 bits, E=3.6e-09
• PF01687: Flavokinase
  • amino acids 184 to 306 (123 residues), 147.2 bits, E=4.1e-47

Best Hits

Swiss-Prot: 80% identical to RIBF_ECOL6: Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 98% identity to pva:Pvag_0076)

MetaCyc: 80% identical to bifunctional riboflavin kinase / FMN adenylyltransferase (Escherichia coli K-12 substr. MG1655)
FAD synthetase. [EC: 2.7.7.2]; Riboflavin kinase. [EC: 2.7.7.2, 2.7.1.26]

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (9/9 steps found)
• flavin biosynthesis III (fungi) (8/9 steps found)
• flavin salvage (2/2 steps found)
• 5,6-dimethylbenzimidazole biosynthesis I (aerobic) (2/3 steps found)
• flavin biosynthesis II (archaea) (6/10 steps found)
• roseoflavin biosynthesis (1/7 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (13/33 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Protein Sequence (309 amino acids)

>IAI47_16150 bifunctional riboflavin kinase/FAD synthetase (Pantoea sp. MT58)
MKFIRGIHNLNEQHRGCVLTIGNFDGVHRGHQALLSRLCEEGLKRHLPVVVMVFEPQPLE
LFAGDKAPARLTRLREKLRYMAEAGVDKVLCVRFDRRFAALSAQRFISDLLVDKLDVKYL
AVGDDFRFGAGRQGDFLLLQKAGVEYGFEVVSTETFCDGGKRISSTAVRQALAADDLALA
QSLLGHPFTISGRVVHGDALGRTLGFPTANLPLRRSVTPVKGVYAVEVQGLTPEPLPGVA
NIGTRPTVKGLRQQLEVHLLDINIDLYGRHIDVVLKQKIRNEQRFASLEALKEQIANDVV
TARQFFGLL