Protein Info for IAI47_16095 in Pantoea sp. MT58

Annotation: bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 285 TIGR00668: bis(5'-nucleosyl)-tetraphosphatase (symmetrical)" amino acids 1 to 277 (277 residues), 498.8 bits, see alignment E=2.2e-154 PF00149: Metallophos" amino acids 2 to 139 (138 residues), 63.2 bits, see alignment E=2.3e-21

Best Hits

Swiss-Prot: 80% identical to APAH_ENT38: Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (apaH) from Enterobacter sp. (strain 638)

KEGG orthology group: K01525, bis(5'-nucleosyl)-tetraphosphatase (symmetrical) [EC: 3.6.1.41] (inferred from 98% identity to pva:Pvag_0089)

MetaCyc: 80% identical to diadenosine tetraphosphatase (Escherichia coli K-12 substr. MG1655)
Bis(5'-nucleosyl)-tetraphosphatase (symmetrical). [EC: 3.6.1.41]; Guanosine-5'-triphosphate,3'-diphosphate diphosphatase. [EC: 3.6.1.41, 3.6.1.40]

Predicted SEED Role

"Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41)" (EC 3.6.1.41)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.40

Use Curated BLAST to search for 3.6.1.40 or 3.6.1.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (285 amino acids)

>IAI47_16095 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH (Pantoea sp. MT58)
MSTYLIGDVHGCYDELRALLQQVDFNPEQDTLWLTGDLVARGPGSLEVLRYVKSLGDAVR
LVLGNHDLHLLAVYAGISRNKPKDRLTPLLEADDADELINWLRRQPLLQVDEEKKLVMGH
AGITPQWDIETAKKCAREVEAVLASDSYPLFLDAMYGDMPNNWSEDLTGLARLRFSTNAL
TRMRYCFPNGQLDMICKDAPESALPPLKPWFNIAGPVARDYTIVFGHWASLEGKGTPEGI
IGLDTGCCWGGTLTLLHWETQRYMVQPSNREKALASSESLAPPAL