Protein Info for IAI47_16060 in Pantoea sp. MT58

Annotation: bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 217 TIGR00005: pseudouridine synthase, RluA family" amino acids 9 to 215 (207 residues), 196.2 bits, see alignment E=3.8e-62 PF00849: PseudoU_synth_2" amino acids 20 to 167 (148 residues), 107.3 bits, see alignment E=4.3e-35

Best Hits

Swiss-Prot: 85% identical to RLUA_SHIFL: Ribosomal large subunit pseudouridine synthase A (rluA) from Shigella flexneri

KEGG orthology group: K06177, ribosomal large subunit pseudouridine synthase A [EC: 5.4.99.12] (inferred from 98% identity to pva:Pvag_0096)

MetaCyc: 85% identical to 23S rRNA pseudouridine746 and tRNA pseudouridine32 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11843 [EC: 5.4.99.29]; RXN-11842 [EC: 5.4.99.29, 5.4.99.28]

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70)" in subsystem Ribosome biogenesis bacterial (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12 or 5.4.99.28 or 5.4.99.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (217 amino acids)

>IAI47_16060 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA (Pantoea sp. MT58)
MEPYNPPREPWLHILYQDAHIMVVNKPSGLLSVPGRLDEHKDSVMTRIQRDFPQAESVHR
LDMATSGVMVVALTKAAERELKRQFREREPSKTYVACIWGHPEKEEGLVDLPLICDWPNR
PKQMVCFENGKAAQTEYQVLEYRADNSTRISLKPITGRSHQLRVHMLALGHPILGDNFYA
HPEAKAMAPRLLLHAESLTITHPAFGNAMTFRQPADF