Protein Info for IAI47_15990 in Pantoea sp. MT58

Annotation: acetolactate synthase 3 large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00118: acetolactate synthase, large subunit, biosynthetic type
  • amino acids 4 to 569 (566 residues), 918 bits, E=9.6e-281
• PF02776: TPP_enzyme_N
  • amino acids 5 to 119 (115 residues), 138.7 bits, E=1.2e-44
• PF00205: TPP_enzyme_M
  • amino acids 196 to 330 (135 residues), 168.7 bits, E=8.3e-54
• PF02775: TPP_enzyme_C
  • amino acids 394 to 545 (152 residues), 177.8 bits, E=1.9e-56

Best Hits

Swiss-Prot: 86% identical to ILVI_ECOLI: Acetolactate synthase isozyme 3 large subunit (ilvI) from Escherichia coli (strain K12)

KEGG orthology group: K01652, acetolactate synthase I/II/III large subunit [EC: 2.2.1.6] (inferred from 99% identity to pva:Pvag_0109)

MetaCyc: 86% identical to acetolactate synthase / acetohydroxybutanoate synthase, catalytic subunit (Escherichia coli K-12 substr. MG1655)
Acetolactate synthase. [EC: 2.2.1.6]; 2.2.1.6 [EC: 2.2.1.6]

Predicted SEED Role

"Acetolactate synthase large subunit (EC 2.2.1.6)" in subsystem Acetoin, butanediol metabolism or Branched-Chain Amino Acid Biosynthesis (EC 2.2.1.6)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-threonine metabolism (15/18 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• superpathway of (R,R)-butanediol biosynthesis (5/5 steps found)
• L-valine biosynthesis (4/4 steps found)
• pyruvate fermentation to (R)-acetoin I (3/3 steps found)
• pyruvate fermentation to (S)-acetoin (3/3 steps found)
• superpathway of 2,3-butanediol biosynthesis (5/6 steps found)
• pyruvate fermentation to (R)-acetoin II (2/2 steps found)
• pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• L-isoleucine biosynthesis III (4/7 steps found)
• L-isoleucine biosynthesis II (4/8 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Butanoate metabolism
• C5-Branched dibasic acid metabolism
• Pantothenate and CoA biosynthesis
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.2.1.6

Use Curated BLAST to search for 2.2.1.6

Protein Sequence (574 amino acids)

>IAI47_15990 acetolactate synthase 3 large subunit (Pantoea sp. MT58)
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALQTVGGIDHILVRHEQGAVHMADG
LARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVILSGQVPSSLIGYDAFQECDMVG
ISRPVVKHSFLVKNTEEIPSVLKKAFWLASSGRPGPVVIDLPKDILNPANKLPYHWPESV
SMRSYNPTIQGHKGQIKRALTTLLAAKKPVIYAGGGAITAGCEAELLQLAESLNIPVTTS
LMGLGAFPGTHRQCVGMLGMHGTYEANMTMHNTDLIFGIGVRFDDRTTNNLAKYCPNATV
MHIDIDPTSISKTVSADIPIVGDAKQVLQQMLELLTQSDTPSRAESLHDWWQEIEGWRSR
HCLEFDRTSDKIKPQAVIETISRLTKGDAYVTSDVGQHQMFAALYYPFDKPRRWINSGGL
GTMGFGLPAALGVKMALPEETVICVTGDGSIQMNIQELSTALQYDLPVLVLNLNNRVLGM
VKQWQDMIYSGRHSQSYMESLPDFVRLAEAYGHVGIAIEHPAELEEKLTLALETLAKGRL
VFVDVTVDGSEHVYPMQIRGGSMDEMWLSKTERT