Protein Info for IAI47_15925 in Pantoea sp. MT58

Annotation: UDP-N-acetylmuramate--L-alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 21 to 119 (99 residues), 118.2 bits, E=2.5e-38
• TIGR01082: UDP-N-acetylmuramate--L-alanine ligase
  • amino acids 21 to 475 (455 residues), 608.9 bits, E=3.4e-187
• PF08245: Mur_ligase_M
  • amino acids 124 to 304 (181 residues), 97.2 bits, E=2e-31
• PF02875: Mur_ligase_C
  • amino acids 339 to 415 (77 residues), 53.9 bits, E=2.9e-18

Best Hits

Swiss-Prot: 87% identical to MURC_SERP5: UDP-N-acetylmuramate--L-alanine ligase (murC) from Serratia proteamaculans (strain 568)

KEGG orthology group: K01924, UDP-N-acetylmuramate--alanine ligase [EC: 6.3.2.8] (inferred from 99% identity to pva:Pvag_0122)

MetaCyc: 85% identical to UDP-N-acetylmuramate--L-alanine ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramate--L-alanine ligase. [EC: 6.3.2.8]

Predicted SEED Role

"UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.8)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.8

Protein Sequence (492 amino acids)

>IAI47_15925 UDP-N-acetylmuramate--L-alanine ligase (Pantoea sp. MT58)
MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGYHISGSDLAPNPVTQNL
IALGATIYFNHRPENVTDASVVVVSSAVSQDNPELVAAREQRIPVIRRAEMLAELMRFRH
GIAVAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAGTHARLGNSRYLIAEADESDA
SFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFLHNLPFYGRAVMCVDDAVIRDLL
PRVGRHITTYGFSDDADLRIENYEQSGAQGHFTLIRQDKAPLQITLNAPGRHNALNAAAA
VAVASEEGIEDNAILAALESFQGTGRRFDLLGEFPTEPVNGQPGTAMLVDDYGHHPTEVD
ATIKAARAGWPDKKLVMVFQPHRYSRTRDLFDDFANVLSHVDVLLMLDVYSAGETAIPGA
DSRSLCRAIRNRGKVDPILVPEHDALPEMLAPLLSGNDLILVQGAGNVGKVARKLADTKL
QPERKAEDRHGG