Protein Info for IAI47_15770 in Pantoea sp. MT58

Annotation: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00117: aconitate hydratase 2
  • amino acids 1 to 854 (854 residues), 1657.5 bits, E=0
• PF11791: Aconitase_B_N
  • amino acids 4 to 156 (153 residues), 234 bits, E=1.1e-73
• PF06434: Aconitase_2_N
  • amino acids 168 to 382 (215 residues), 327.9 bits, E=3.6e-102
• PF00330: Aconitase
  • amino acids 472 to 818 (347 residues), 134.2 bits, E=1.1e-42

Best Hits

Swiss-Prot: 92% identical to ACNB_SALTY: Aconitate hydratase B (acnB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01682, aconitate hydratase 2 [EC: 4.2.1.3] (inferred from 82% identity to aha:AHA_3856)

MetaCyc: 92% identical to AcnB (Escherichia coli K-12 substr. MG1655)
2-methylisocitrate dehydratase. [EC: 4.2.1.99]; Aconitate hydratase. [EC: 4.2.1.99, 4.2.1.3]; 4.2.1.3 [EC: 4.2.1.99, 4.2.1.3]

Predicted SEED Role

"Aconitate hydratase 2 (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or TCA Cycle (EC 4.2.1.3)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• mixed acid fermentation (14/16 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle III (animals) (8/10 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (6/9 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• nitrogen remobilization from senescing leaves (5/8 steps found)
• 2-methylcitrate cycle I (2/5 steps found)
• reductive TCA cycle I (6/11 steps found)
• 2-methylcitrate cycle II (2/6 steps found)
• reductive TCA cycle II (5/12 steps found)
• methylaspartate cycle (9/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3

Use Curated BLAST to search for 4.2.1.3 or 4.2.1.99

Protein Sequence (865 amino acids)

>IAI47_15770 bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase (Pantoea sp. MT58)
MLEEYRKHVAERAAQGIVPKPLEASQMAALVELLKNPPAGEEEFLTDLLVNRVPPGVDEA
AYVKAGFLAAVAKGEATSPLISPEKAVELLGTMQGGYNIHPLIEALDDSKLAPIAAKALS
HTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWYLSRPELAEKITVTVFKVTGETNTD
DLSPAQDAWSRPDIPLHALAMLKNAREGIEPDEAGNIGPIKQIEALKTKGFPLAYVGDVV
GTGSSRKSATNSVLWFMGDDIPNVPNKKGGGVVLGSKIAPIFFNTMEDAGALPIEVDVNN
LNMGDVIDIYPFKGEVRNHETNELLASFELKTEVVIDEVRAGGRIPLIIGRGLTTKARES
LGLPHSTVFRQAKDVAESTRGFSLAQKMVGRACGVAGVRPGAYCEPKMTSVGSQDTTGPM
TRDELKDLACLGFSTDLVMQSFCHTAAYPKPVDVSTHHTLPDFIMNRGGVSLRPGDGIIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKM
EPGITLRDLVHAIPLYAIKQGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELSDASA
ERSAAGCTIKLDKAPIIEYLNSNIVLLKWMIAEGYGDRRTLERRVESMQKWLADPQLLEA
DADAEYAAVIEIDLADIKEPILCAPNDPDDARLLSDVQGEKIDEVFIGSCMTNIGHFRAA
GKLLDSHKGQLPTRLWVAPPTKMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVA
DGATVVSTSTRNFPNRLGNGANVYLASAELAAVASLLGKLPTPDEYQQFMEQVDKTASDT
YRYLNFDQLAQYTDKADGVIFQTAV