Protein Info for IAI47_15435 in Pantoea sp. MT58

Annotation: tRNA lysidine(34) synthetase TilS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 439 PF01171: ATP_bind_3" amino acids 16 to 193 (178 residues), 206.1 bits, see alignment E=6.1e-65 TIGR02432: tRNA(Ile)-lysidine synthetase" amino acids 16 to 197 (182 residues), 177 bits, see alignment E=3.8e-56 PF09179: TilS" amino acids 242 to 309 (68 residues), 63.1 bits, see alignment E=3.7e-21 TIGR02433: tRNA(Ile)-lysidine synthetase, C-terminal domain" amino acids 358 to 403 (46 residues), 53.5 bits, see alignment 1.3e-18 PF11734: TilS_C" amino acids 358 to 429 (72 residues), 84 bits, see alignment E=5.7e-28

Best Hits

Swiss-Prot: 58% identical to TILS_SERP5: tRNA(Ile)-lysidine synthase (tilS) from Serratia proteamaculans (strain 568)

KEGG orthology group: K04075, tRNA(Ile)-lysidine synthase [EC: 6.3.4.-] (inferred from 87% identity to pva:Pvag_0221)

Predicted SEED Role

"tRNA(Ile)-lysidine synthetase (EC 6.3.4.19)" (EC 6.3.4.19)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.- or 6.3.4.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (439 amino acids)

>IAI47_15435 tRNA lysidine(34) synthetase TilS (Pantoea sp. MT58)
MPLPAFDAMLQPDAALVVAFSGGLDSTVLLHQLRGWQQQHPESHLRALHVHHGLSPNADC
WAAHCLSVCEQWQLPCEVLHVTVDGRENGIEAAARTARYQALSAALLPGEVLLTAQHLDD
QCETFMLALKRGSGPAGLAAMPAARALGSHQLLRPLLNQSRQSLETYARTHQLVWIEDES
NQDLRYDRNFLRQRLLPALYQRWPHFAEATARSAALCSEQEQLLDELLTGQLNELIQPDG
SLHFPPLMVMSELRANALLRRWIAGQGGRMPSREALKRIRDEVMASRGDAQPKLRFGQAE
LRRYRQQLYWLPLRSSLRYTELAWPDLCQPLVLPQNLGTLCASQAQSLLRYPQPDEQVSV
RFHAQGHVHLVGRQGGREMKKLWQELQIPPWQRERLPLIFYNQTLICVPNLFVTHAGAAV
DQQGCQIVWDRACSAEEQQ