Protein Info for IAI47_14425 in Pantoea sp. MT58
Annotation: bifunctional UDP-sugar hydrolase/5'-nucleotidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 59% identical to USHA_YERE8: Protein UshA (ushA) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
KEGG orthology group: K11751, 5'-nucleotidase / UDP-sugar diphosphatase [EC: 3.1.3.5 3.6.1.45] (inferred from 92% identity to pva:Pvag_0420)MetaCyc: 57% identical to 5'-nucleotidase / UDP-sugar hydrolase (Escherichia coli K-12 substr. MG1655)
UDP-sugar diphosphatase. [EC: 3.6.1.45]; Bis(5'-adenosyl)-triphosphatase. [EC: 3.6.1.45, 3.6.1.29]; RXN0-3741 [EC: 3.6.1.45, 3.6.1.29, 3.1.3.89]; 5'-nucleotidase. [EC: 3.6.1.45, 3.6.1.29, 3.1.3.89, 3.1.3.5]; 4-nitrophenylphosphatase. [EC: 3.6.1.45, 3.6.1.29, 3.1.3.89, 3.1.3.5, 3.1.3.41]; RXN-18241 [EC: 3.6.1.45, 3.6.1.29, 3.1.3.89, 3.1.3.5, 3.1.3.41]
Predicted SEED Role
"UDP-sugar hydrolase (EC 3.6.1.45); 5'-nucleotidase (EC 3.1.3.5)" (EC 3.1.3.5, EC 3.6.1.45)
MetaCyc Pathways
- purine nucleotides degradation II (aerobic) (11/11 steps found)
- ureide biosynthesis (7/7 steps found)
- adenosine nucleotides degradation II (5/5 steps found)
- purine nucleotides degradation I (plants) (10/12 steps found)
- adenosine nucleotides degradation I (7/8 steps found)
- guanosine nucleotides degradation II (4/4 steps found)
- guanosine nucleotides degradation III (4/4 steps found)
- inosine 5'-phosphate degradation (4/4 steps found)
- NAD salvage pathway III (to nicotinamide riboside) (3/3 steps found)
- superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
- guanosine nucleotides degradation I (3/4 steps found)
- superpathway of purines degradation in plants (13/18 steps found)
- UTP and CTP dephosphorylation I (5/7 steps found)
- NAD salvage (plants) (5/11 steps found)
- tunicamycin biosynthesis (2/9 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Nicotinate and nicotinamide metabolism
- Purine metabolism
- Pyrimidine metabolism
- gamma-Hexachlorocyclohexane degradation
Isozymes
Compare fitness of predicted isozymes for: 3.1.3.5
Use Curated BLAST to search for 3.1.3.41 or 3.1.3.5 or 3.1.3.89 or 3.6.1.29 or 3.6.1.45
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (588 amino acids)
>IAI47_14425 bifunctional UDP-sugar hydrolase/5'-nucleotidase (Pantoea sp. MT58) MLLSKWTGVGMALLVAVMPLFSQAAQADRTYRFSLLHTNDEHGHFWFNEQGEYGLAAQKT LMDTMRYDVQSRGGGALILSAGDVNTGVPESDELDAEPDFRAMNLIGYDAMALGNHEFDK PIAVLRKQEQWARFPLLAANVYQKSTGQRLFKPWAVFNRLGLKIAVVGLITPDTAKLANP AGLTDTEFRDPVAETVKAVAELRATEKPDVIVALTHLGHYDDGKHGSNGIGDVTLARSLP AGTIDLIVGGHSHDAVCMEKENVSVKNYQPGQPCEPDKQNGIWIMQADKWGKYVGRGDFT FRNGQVTLEAYQLVPVNLKHKIKNADGSETWLTWQEEIPKNTAMMKLLMPFQKRAEAKLI VNVGSADGNFSGEKEQVRFEQTNLGQLILRAQMAFTKADFAVMSGGGIRTSLKAGKLNWR DLLQVQPFGNQVVSVTLTGAEIKKYLDVVANIQPDSGGYAQFAGISLTTDGKQVSNVSIN GKPLQDTQIYRMATNSFNAAGGDGYPRLDNHPGYANSGAVDAQVLRDFIKAHSPVKASDY QPKEQIVHLTDEQIRARDEAAEKARKRNYPKMILAWIWPWGNETARTH