Protein Info for IAI47_13515 in Pantoea sp. MT58

Annotation: ATP-dependent dethiobiotin synthetase BioD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13500: AAA_26
  • amino acids 2 to 210 (209 residues), 136.1 bits, E=1.4e-43
• PF01656: CbiA
  • amino acids 5 to 212 (208 residues), 53.4 bits, E=2.6e-18
• TIGR00347: dethiobiotin synthase
  • amino acids 5 to 175 (171 residues), 200.3 bits, E=1.2e-63

Best Hits

Swiss-Prot: 68% identical to BIOD1_ECOLI: ATP-dependent dethiobiotin synthetase BioD 1 (bioD1) from Escherichia coli (strain K12)

KEGG orthology group: K01935, dethiobiotin synthetase [EC: 6.3.3.3] (inferred from 96% identity to pva:Pvag_0592)

MetaCyc: 68% identical to dethiobiotin synthetase (Escherichia coli K-12 substr. MG1655)
Dethiobiotin synthase. [EC: 6.3.3.3]

Predicted SEED Role

"Dethiobiotin synthetase (EC 6.3.3.3)" in subsystem Biotin biosynthesis (EC 6.3.3.3)

MetaCyc Pathways

• biotin biosynthesis I (14/15 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate I (4/4 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate II (3/4 steps found)
• biotin biosynthesis II (4/6 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate III (3/5 steps found)

KEGG Metabolic Maps

• Biotin metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.3.3

Use Curated BLAST to search for 6.3.3.3

Protein Sequence (223 amino acids)

>IAI47_13515 ATP-dependent dethiobiotin synthetase BioD (Pantoea sp. MT58)
MKRFFITGTDTEVGKTVASGALLQAAATAGFRCAGYKPVASGCEMTPEGIRNSDALALQR
FSSVPLTYQQVNPLAFMEPTSPHIVSAEEGRTITHLALSAGLAALEPLADWILVEGAGGW
YTPLSETHTYADWVVSEKLPVILVVGIKLGCINHAMLTAEAVRARGLPLVGWIANTVEPP
GKRYPEYLTSLKNRLPAPCLGTIPYLTDAAQQASCGHYLTLPA