Protein Info for IAI47_13285 in Pantoea sp. MT58

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01891: amidohydrolase
  • amino acids 13 to 370 (358 residues), 343.1 bits, E=1e-106
• PF01546: Peptidase_M20
  • amino acids 69 to 380 (312 residues), 150.1 bits, E=8.4e-48
• PF07687: M20_dimer
  • amino acids 180 to 274 (95 residues), 50.6 bits, E=1.7e-17

Best Hits

Swiss-Prot: 37% identical to YHAA_BACSU: Putative amidohydrolase YhaA (yhaA) from Bacillus subtilis (strain 168)

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 97% identity to pva:Pvag_0643)

MetaCyc: 37% identical to N-acetyl amino acid acetylase (Bacillus subtilis subtilis 168)
3.5.1.-

Predicted SEED Role

"Metal-dependent amidase/aminoacylase/carboxypeptidase"

MetaCyc Pathways

• S-(2-succinyl)-L-cysteine degradation (2/4 steps found)
• S-benzyl-L-cysteine degradation (2/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32

Use Curated BLAST to search for 3.5.1.32

Protein Sequence (385 amino acids)

>IAI47_13285 amidohydrolase (Pantoea sp. MT58)
MTLSPSLLEDALHWRREFHRQPELGYQEQQTSQFIAEELARAGLQVFRGLAGTGVIGTLE
NGSGPVIGLRADMDALPITEKGDAEWRSSTPGVMHACGHDGHSAILLAAARQLAATRQFS
GTVHFIFQPAEENLGGARKMVEEGLFQRFPMDAIYAMHNWPGLPLGSLAVNPGAMMASLD
SFEITLHGKSCHAAMPESGADPMVVAAELILALQTIPSRRLSPLASAVVSVTQIYGGEAI
NVIPEQIMLRGTVRCLQTSVREKVRGLIDEFVTTLPRPFGVSGEINWLPGYPVTANHVAP
AEQVRDVALATLGAEQVHWQVNPSMASEDFACMMEACPGAYFWLGADGETPSAPLHNARY
DFNDALLPIGIRFWQNLVEQALPAA