Protein Info for IAI47_12980 in Pantoea sp. MT58

Annotation: N-acetylmuramoyl-L-alanine amidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01510: Amidase_2
  • amino acids 40 to 176 (137 residues), 118.6 bits, E=1.2e-38

Best Hits

Swiss-Prot: 62% identical to AMID_ECOLI: N-acetylmuramoyl-L-alanine amidase AmiD (amiD) from Escherichia coli (strain K12)

KEGG orthology group: K11066, N-acetylmuramoyl-L-alanine amidase [EC: 3.5.1.28] (inferred from 94% identity to pva:Pvag_0710)

MetaCyc: 62% identical to N-acetylmuramoyl-L-alanine amidase D (Escherichia coli K-12 substr. MG1655)
N-acetylmuramoyl-L-alanine amidase. [EC: 3.5.1.28]

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)" (EC 3.5.1.28)

MetaCyc Pathways

• peptidoglycan recycling I (14/14 steps found)
• peptidoglycan recycling II (7/10 steps found)

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Protein Sequence (274 amino acids)

>IAI47_12980 N-acetylmuramoyl-L-alanine amidase (Pantoea sp. MT58)
MKRGLLLILALLLAGCHSGIESRDGYWADLRHPAQGARPRVKVIVIHYTAEDFSSSLATL
TDREVSVHYLIPRQPPQRAGKGIVWQLVPEQQLAWHAGPSFWRGASRINDTSIGIELVNR
GYRRTLTGLEWQPFTAAQIITLEALVRDIAQRYGIPPENIVGHSDIAPQRKQDPGPLFPW
HQLAQRGLGAWPDAGRVQFYLAGQDPHAQVPVQPLMENLQRFGYAATAEMSAREQQKVIA
AFQMHFRPADYRGQPDAETAAIAAALLEKYGAAE