Protein Info for IAI47_12880 in Pantoea sp. MT58

Annotation: formate C-acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01255: formate acetyltransferase
  • amino acids 11 to 760 (750 residues), 1393.9 bits, E=0
• PF02901: PFL-like
  • amino acids 24 to 614 (591 residues), 709.8 bits, E=4.1e-217
• PF01228: Gly_radical
  • amino acids 631 to 741 (111 residues), 124.5 bits, E=2.5e-40

Best Hits

Swiss-Prot: 90% identical to PFLB_ECOLI: Formate acetyltransferase 1 (pflB) from Escherichia coli (strain K12)

KEGG orthology group: K00656, formate C-acetyltransferase [EC: 2.3.1.54] (inferred from 91% identity to dda:Dd703_2243)

MetaCyc: 90% identical to pyruvate formate-lyase (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Pyruvate formate-lyase (EC 2.3.1.54)" in subsystem Butanol Biosynthesis or Fermentations: Mixed acid (EC 2.3.1.54)

MetaCyc Pathways

• hexitol fermentation to lactate, formate, ethanol and acetate (17/19 steps found)
• mixed acid fermentation (14/16 steps found)
• superpathway of N-acetylneuraminate degradation (18/22 steps found)
• superpathway of L-threonine metabolism (15/18 steps found)
• L-threonine degradation I (6/6 steps found)
• pyruvate fermentation to acetate IV (3/3 steps found)
• pyruvate fermentation to ethanol I (3/3 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (7/9 steps found)
• reductive monocarboxylic acid cycle (1/2 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (5/13 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Propanoate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.54

Use Curated BLAST to search for 2.3.1.54

Protein Sequence (760 amino acids)

>IAI47_12880 formate C-acetyltransferase (Pantoea sp. MT58)
MTELNAKLASAWEGFAEGEWQNSVNVRDFIQKNYTPYEGDESFLAGATEATTKLWESVLE
GIKIENRTHAPVDFDTDLASTITSHDAGYINKSLEKIVGLQTEAPLKRALIPFGGIKMVE
GSCKVYGRELDPSLKKIFTEYRKTHNQGVFDVYTPDIMRCRKSGILTGLPDAYGRGRIIG
DYRRVALYGIDFLMADKFSQFASLQNDLENGMNLEATIRLREEISDQHRALAQIKEMAAK
YGHDISGPATNAVEAVQWTYYGYLAAVKSQNGAAMSFGRVSTFLDVYIERDLKAGKLTEE
QAQELIDHLVMKLRMVRFLRTPEYDELFSGDPIWATESLAGMGVDGRTLVSKSTFRFLNT
LYTMGPSPEPNMTILWSEKLPINFKKYAAKVSIDTSSLQYENDDLMRPDFDNDDYAIACC
VSPMIVGKQMQFFGARANLAKTLLYAINGGVDEKLKMQVGPKGDKISDDVLDFDTVMERM
DHFMDWLAKQYVTSLNIIHYMHDKYSYEAALMALHDRDVYRTMACGIAGLSVAADSLSAI
KYAKVKPIRDEDGLAIDFDIEGEYPQFGNNDSRVDDLACDLVERFMKKIQQLTTYRNAVP
TQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYA
KDGISYTFSIVPNALGKDDNVRKTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAM
EHPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTQSL