Protein Info for IAI47_12830 in Pantoea sp. MT58

Annotation: tetraacyldisaccharide 4'-kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02606: LpxK
  • amino acids 13 to 317 (305 residues), 413.4 bits, E=3.1e-128
• TIGR00682: tetraacyldisaccharide 4'-kinase
  • amino acids 19 to 322 (304 residues), 354.6 bits, E=2.3e-110

Best Hits

Swiss-Prot: 70% identical to LPXK_PECCP: Tetraacyldisaccharide 4'-kinase (lpxK) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K00912, tetraacyldisaccharide 4'-kinase [EC: 2.7.1.130] (inferred from 92% identity to pva:Pvag_0741)

MetaCyc: 64% identical to tetraacyldisaccharide 4'-kinase (Escherichia coli K-12 substr. MG1655)
Tetraacyldisaccharide 4'-kinase. [EC: 2.7.1.130]

Predicted SEED Role

"Tetraacyldisaccharide 4'-kinase (EC 2.7.1.130)" in subsystem KDO2-Lipid A biosynthesis or Lipopolysaccharide-related cluster in Alphaproteobacteria (EC 2.7.1.130)

MetaCyc Pathways

• superpathway of (Kdo)₂-lipid A biosynthesis (17/17 steps found)
• lipid IV_A biosynthesis (P. gingivalis) (9/9 steps found)
• lipid IV_A biosynthesis (E. coli) (6/6 steps found)
• lipid IV_A biosynthesis (H. pylori) (6/6 steps found)
• lipid IV_A biosynthesis (P. putida) (6/6 steps found)
• lipid IV_A biosynthesis (Vibrio cholerae serogroup O1 El Tor) (6/6 steps found)
• lipid IV_A biosynthesis (generic) (6/6 steps found)
• superpathway of Kdo₂-lipid A biosynthesis (19/25 steps found)
• lipid IV_A biosynthesis (2,3-diamino-2,3-dideoxy-D-glucopyranose-containing) (5/6 steps found)

KEGG Metabolic Maps

• Lipopolysaccharide biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.130

Protein Sequence (328 amino acids)

>IAI47_12830 tetraacyldisaccharide 4'-kinase (Pantoea sp. MT58)
MIERIWSGRSPLWLLLWPFSLLYGAITALIRFSYRRGWRKSWRAPLPVVVVGNLTAGGNG
KTPVVIWLVEALQQRGLRAGVVSRGYGGKADHYPLLVTAQTSTEQAGDEPVLIAQRTGAP
VAVAPRRRQAIEALLQQGSLDVVITDDGLQHYALQRDREIVVVDGVRRFGNGWWLPAGPM
RERASRLRQVDAVIINGGDAQPDEIAMQLQPGLATNLLTGETCPAEQLGAVVAMAGIGHP
PRFFTTLKKQGITPVAEIAFADHHAYSEDELTRLLQTGQQLLMTEKDAVKCRDFAQPDWW
YLPVDAHLTGKSVTPLLADIEQLCRRTL