Protein Info for IAI47_12745 in Pantoea sp. MT58

Annotation: aminopeptidase N

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 871 TIGR02414: aminopeptidase N" amino acids 12 to 869 (858 residues), 1249.3 bits, see alignment E=0 PF17900: Peptidase_M1_N" amino acids 88 to 186 (99 residues), 54 bits, see alignment E=4.8e-18 PF01433: Peptidase_M1" amino acids 225 to 439 (215 residues), 195.1 bits, see alignment E=2.6e-61 PF11940: DUF3458" amino acids 444 to 545 (102 residues), 108 bits, see alignment E=5.3e-35 PF17432: DUF3458_C" amino acids 548 to 870 (323 residues), 402.1 bits, see alignment E=4.2e-124

Best Hits

Swiss-Prot: 80% identical to AMPN_ECOLI: Aminopeptidase N (pepN) from Escherichia coli (strain K12)

KEGG orthology group: K01256, aminopeptidase N [EC: 3.4.11.2] (inferred from 85% identity to ebi:EbC_15240)

MetaCyc: 80% identical to aminopeptidase N (Escherichia coli K-12 substr. MG1655)
Cytosol nonspecific dipeptidase. [EC: 3.4.13.18]; Cytosol alanyl aminopeptidase. [EC: 3.4.13.18, 3.4.11.14, 3.4.11.2]; 3.4.11.- [EC: 3.4.13.18, 3.4.11.14, 3.4.11.2]

Predicted SEED Role

"Membrane alanine aminopeptidase N (EC 3.4.11.2)" (EC 3.4.11.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.11.14 or 3.4.11.2 or 3.4.13.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (871 amino acids)

>IAI47_12745 aminopeptidase N (Pantoea sp. MT58)
MTQQPQIKYRHDYRAPDYTITDIDLTFELDASTTRVTAISQVKRLGDSQAELQLDGEALT
LIALEIDGQPWTAYREEEGALVIGQLPESFTLKIINDIHPDQNTALEGLYKSGEALCTQC
EAEGFRHITWYLDRPDVLARFTTTIYADGERYPFLLSNGNRIDGGRDEQGRSWVKWEDPF
PKPCYLFALVAGDFDVLRDSFTTRSGRDVALEIFVDRGNLDRADWAMTSLKNSMKWDEER
FGLEYDLDIFMIVAVDFFNMGAMENKGLNVFNAKYVLAKAETATDKDYLGIEAVIGHEYF
HNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDLGSRAVNRIDNVRIVRGAQFAEDASPM
AHPIRPDQVIEMNNFYTLTVYEKGSEVIRMMHTLLGEENFQKGMKLYFDRHDGSAATCDD
FVQAMEDASNVDLSQFRRWYSQSGTPVLTVRDDYNPELEQYTLHVTQHTPATADQKEKLP
LHIPLDIELYDGEGKVIPLQNNGHPVHHVLNVTEEFQTFIFDNVYFQPVPSLLREFSAPV
KLDYKWSDAQLTFLMRHARNDFSRWDAAQSLLATYIRLNVARYQQGQHLSLPLHVADAFR
AVLLDTDCDPALTALILTLPSENEIAELFDTIDPQAIALVRDALQRTLARELADEFFAIY
NANQQPEYRVEHDAIGQRSLKNTCLTYLAFSDAQLADKLVQTQYHQATNMTDALAALSAA
VAAQLPCRDALLAAYDERWHQDGLVMDKWFVLQATSPAANVMRNVRQLLTHRSFTMSNPN
RVRSLVGAFASANPAAFHAKDGSGYKFLVEMLTDLNTRNPQVAARMVEPLIRLKRYDEDR
QAMMRQALEQLLQLDKLSGDLYEKITRALNA