Protein Info for IAI47_12720 in Pantoea sp. MT58

Annotation: NADPH-dependent FMN reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03358: FMN_red
  • amino acids 1 to 141 (141 residues), 110.8 bits, E=4.8e-36
• PF02525: Flavodoxin_2
  • amino acids 1 to 92 (92 residues), 27.1 bits, E=3.2e-10
• TIGR03567: FMN reductase
  • amino acids 2 to 169 (168 residues), 227.3 bits, E=5.6e-72

Best Hits

Swiss-Prot: 67% identical to SSUE_ECOLI: FMN reductase (NADPH) (ssuE) from Escherichia coli (strain K12)

KEGG orthology group: K00299, FMN reductase [EC: 1.5.1.29] (inferred from 96% identity to pva:Pvag_0763)

MetaCyc: 67% identical to NADPH-dependent FMN reductase (Escherichia coli K-12 substr. MG1655)
RXN-12444 [EC: 1.5.1.38]

Predicted SEED Role

"FMN reductase (EC 1.5.1.29)" in subsystem Alkanesulfonate assimilation or Alkanesulfonates Utilization (EC 1.5.1.29)

MetaCyc Pathways

• two-component alkanesulfonate monooxygenase (2/2 steps found)
• 5,6-dimethylbenzimidazole biosynthesis I (aerobic) (2/3 steps found)
• bacterial bioluminescence (4/8 steps found)
• dibenzothiophene desulfurization (1/5 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (13/33 steps found)

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.29

Use Curated BLAST to search for 1.5.1.29 or 1.5.1.38

Protein Sequence (183 amino acids)

>IAI47_12720 NADPH-dependent FMN reductase (Pantoea sp. MT58)
MRVITLAGSPRFPSRSTSLLTLCQHKLEARGIEVIPWNIHNFQPDDLINARFDAPALLAL
REDLASADGLIVATPVYKASFSGALKTLLDLLPERALEHKVVLPLASGGSVGHMLALDYA
LKPVLNALKAQEILHGVYAEDSQITHYDRQPVLQDSLSQRLDEAVTAFWLALTRHHHPVA
QAV