Protein Info for IAI47_12705 in Pantoea sp. MT58

Annotation: quinone-dependent dihydroorotate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01036: dihydroorotate dehydrogenase (fumarate)
  • amino acids 3 to 336 (334 residues), 500 bits, E=1.4e-154
• PF01180: DHO_dh
  • amino acids 47 to 335 (289 residues), 281.4 bits, E=4.1e-88

Best Hits

Swiss-Prot: 81% identical to PYRD_ERWT9: Dihydroorotate dehydrogenase (quinone) (pyrD) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 97% identity to pva:Pvag_0767)

MetaCyc: 78% identical to dihydroorotate dehydrogenase, type 2 (Escherichia coli K-12 substr. MG1655)
Dihydroorotate dehydrogenase. [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]

Predicted SEED Role

"Dihydroorotate dehydrogenase (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.3.1 or 1.3.5.2 or 1.3.98.1

Protein Sequence (336 amino acids)

>IAI47_12705 quinone-dependent dihydroorotate dehydrogenase (Pantoea sp. MT58)
MFYPIVRPALFKLDPERAHELTFQQLRFMSGTPLEMFYRQTLPSRPVTCMGLTFKNALGL
AAGLDKNAECIDAFAAMGFGFVEVGTVTPRPQAGNDKPRMFRLVEAGGIINRMGFNNLGV
DHLVENVKKARFNGVLGINIGKNKDTPVEQGKDDYLICMEKVYAHAGYIAINISSPNTPG
LRSLQYGEALDDLLSSIKQKQKELEQRHLKYVPLAVKIAPDLSEEELIQVADSLIRHQID
GVIACNTTLDRSLVSGLKYAEEAGGLSGRPVQSRSTAVIQRLSQELQGRLPIIGVGGIDS
LTAAREKIAAGATLVQIYSGFIYQGPGLIKDIVTNI