Protein Info for IAI47_12595 in Pantoea sp. MT58

Annotation: 23S rRNA (cytosine(1962)-C(5))-methyltransferase RlmI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF17785: PUA_3
  • amino acids 5 to 68 (64 residues), 74.6 bits, E=1.1e-24
• PF10672: Methyltrans_SAM
  • amino acids 185 to 371 (187 residues), 100.4 bits, E=2.7e-32
• PF03602: Cons_hypoth95
  • amino acids 217 to 302 (86 residues), 39.2 bits, E=1.6e-13
• PF13847: Methyltransf_31
  • amino acids 223 to 355 (133 residues), 43.2 bits, E=8.8e-15
• PF05175: MTS
  • amino acids 244 to 352 (109 residues), 23.9 bits, E=7.1e-09

Best Hits

Swiss-Prot: 88% identical to RLMI_ERWT9: Ribosomal RNA large subunit methyltransferase I (rlmI) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K06969, ribosomal RNA large subunit methyltransferase I [EC: 2.1.1.-] (inferred from 98% identity to pva:Pvag_0789)

MetaCyc: 84% identical to 23S rRNA m5C1962 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11602 [EC: 2.1.1.191]

Predicted SEED Role

"LSU m5C1962 methyltransferase RlmI" in subsystem Ribosome biogenesis bacterial

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.191

Protein Sequence (396 amino acids)

>IAI47_12595 23S rRNA (cytosine(1962)-C(5))-methyltransferase RlmI (Pantoea sp. MT58)
MTVRLILAKGREKSLLRRHPWVFSGAVARLEGKAQRGETLDVCDSNGKWLARAAYSPESQ
IRARVWSWQADESIDIAFFVRRFEQAQQWRKWLAARDGLDSYRLIAGESDNLPGVTIDRF
GNFLVMQLLSAGAEYQRAAIITALQQCFPGCAIYDRSDVAVRKKEGLELTQGPVTGELPP
PLLPITEHGMKLLVDIQGGHKTGYYLDQRDSRLATRRYAQDARVLNCFSYTGGFAVSALM
GGCKEVISVDTSQEALDVARQNVELNELDLSRARFERDDVFKLLRRYRDSGEKFDLIIMD
PPKFVENKSQLMGACRGYKDINMLAIQLLNPGGVLMTFSCSGLMATELFQKIIADAALDA
GREVQFIEQFRQAADHPVIASYPEGLYLKGFACRVM