Protein Info for IAI47_09565 in Pantoea sp. MT58

Annotation: Re/Si-specific NAD(P)(+) transhydrogenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 510 transmembrane" amino acids 169 to 191 (23 residues), see Phobius details amino acids 403 to 420 (18 residues), see Phobius details amino acids 426 to 443 (18 residues), see Phobius details amino acids 453 to 471 (19 residues), see Phobius details amino acids 477 to 499 (23 residues), see Phobius details TIGR00561: NAD(P)(+) transhydrogenase (AB-specific), alpha subunit" amino acids 3 to 510 (508 residues), 932.8 bits, see alignment E=2.6e-285 PF05222: AlaDh_PNT_N" amino acids 4 to 137 (134 residues), 161.2 bits, see alignment E=2.5e-51 PF01262: AlaDh_PNT_C" amino acids 141 to 368 (228 residues), 237.9 bits, see alignment E=1.2e-74 PF12769: PNTB_4TM" amino acids 427 to 509 (83 residues), 119.6 bits, see alignment E=9.4e-39

Best Hits

Swiss-Prot: 81% identical to PNTA_ECOLI: NAD(P) transhydrogenase subunit alpha (pntA) from Escherichia coli (strain K12)

KEGG orthology group: K00324, NAD(P) transhydrogenase subunit alpha [EC: 1.6.1.2] (inferred from 94% identity to pva:Pvag_1423)

MetaCyc: 81% identical to pyridine nucleotide transhydrogenase subunit alpha (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-277 [EC: 7.1.1.1]

Predicted SEED Role

"NAD(P) transhydrogenase alpha subunit (EC 1.6.1.2)" in subsystem Phosphate metabolism (EC 1.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.1.2

Use Curated BLAST to search for 1.6.1.2 or 7.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (510 amino acids)

>IAI47_09565 Re/Si-specific NAD(P)(+) transhydrogenase subunit alpha (Pantoea sp. MT58)
MLIGIPKERLPNESRVAATPKTVEQLIRLGFSVTVERDAGKRASFDNESYIAAGATLSDR
DQVWQSDIVLKVNAPDDEEIALTRAGSTLVSFIWPAQHPDLLSKLAARQVTVMAMDSVPR
ISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAA
IGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSEAFIKAEMA
LFASQAREVDIIVTTALIPGKPAPKLITEEMVASMKPGSVIVDLAAQTGGNCALTVADQV
TVTPDGVKIIGYTDLPSRLATQSSQLYGTNLVNLMTLLCKEKNGEIQVDFDDVVVRGVTV
VREGEVTWPAPPIQVSAAPQAAPATAKPAVKIEVKPVSPWRKYLLIALAIVLFGSLANVA
PADFLSHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVIGAVLQMGHGGWVTF
ISFIAVLIASINIFGGFTVTQRMLKMFRKG