Protein Info for IAI47_09520 in Pantoea sp. MT58

Annotation: ATP-dependent RNA helicase HrpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 TIGR01967: RNA helicase HrpA" amino acids 14 to 1297 (1284 residues), 2170.4 bits, see alignment E=0 PF00270: DEAD" amino acids 82 to 233 (152 residues), 36.7 bits, see alignment E=9.5e-13 PF00271: Helicase_C" amino acids 282 to 403 (122 residues), 45.3 bits, see alignment E=2.3e-15 PF21010: HA2_C" amino acids 499 to 558 (60 residues), 42.4 bits, see alignment 2.1e-14 PF07717: OB_NTP_bind" amino acids 623 to 698 (76 residues), 58.1 bits, see alignment 2.1e-19 PF11898: DUF3418" amino acids 713 to 1297 (585 residues), 797.8 bits, see alignment E=1.7e-243

Best Hits

Swiss-Prot: 82% identical to HRPA_ECOLI: ATP-dependent RNA helicase HrpA (hrpA) from Escherichia coli (strain K12)

KEGG orthology group: K03578, ATP-dependent helicase HrpA [EC: 3.6.4.13] (inferred from 69% identity to aat:D11S_1569)

MetaCyc: 82% identical to ATP-dependent 3'->5' RNA helicase HrpA (Escherichia coli K-12 substr. MG1655)
5.6.2.e [EC: 5.6.2.e]

Predicted SEED Role

"ATP-dependent RNA helicase HrpA (EC 3.6.4.13)" (EC 3.6.4.13)

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13 or 5.6.2.e

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1300 amino acids)

>IAI47_09520 ATP-dependent RNA helicase HrpA (Pantoea sp. MT58)
MSSPDTSPLAALWSRLDGLMLRDRQRLQRRLQGARKIKNPEAQQGIASELALEFVAAEQR
VAQRRAATPAITFPDNLPVSQKQQDIAEAIRDHQVVIVAGETGSGKTTQLPKICLALGRG
VTGLIGHTQPRRLAARTVANRIADELSTSLGGCVGYKVRFNDQVSDLTQIKLMTDGILLA
EIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLRELLPRRPDLKVIITSATIDPQRFSRH
FNNAPVIEVSGRTYPVEVRYRPVVEEADDGDRDQLQAIFDAVDELGRESRGDILIFMSGE
REIRDTADALNKRDLPHTEVLPLYARLSNAEQNRVFQSHSGRRIVLATNVAETSLTVPGI
KYVIDPGTARISRYSFRTKVQRLPIEPISQASANQRKGRCGRVSDGICIRLYAEDDFLSR
PAFTDPEILRTNLASVILQMTSLGLGDIAAFPFVEAPDKRNIQDGVRLLEELGALTLNEE
NGHYKLTGSGRQLAQLPVDPRLARMVLEAQKFGCVREVMIITAGLSIQDPRERPADKKQA
SDEKHRRFADKDSDFLAYVNLWNYLAEQQKALSGNVFRRQCKTDFLNYLRVREWQDIYTQ
LRQVVRELGIPVNSEPAELQPVHCALLSGLLSHIGQKDADKQEYTGARNTRFAIFPGSGL
FKKPPKWAMVAELVETSRLWGRTAGRIEPEWIEPLAQHLIKRSYSEPHWEKSQGAVMAAE
KVTLYGLPIVAARKVNYGSIDPTLSRELFIRHALVEGDWQTRHAFFRANQKLRSEVEDLE
HKSRRRDILVDDETLFAFYDQRIGKEVVSAKHFDSWWKQASRENAELLNFDKHMLIKEGA
DKVSQLDYPNFWHQGNLKLRLSYQFEPGADADGVTVHIPLPLLNQVEDSGFEWQIPGVRR
ELIIALIKSLPKPLRRNLVPAPNYAEAFLGRVKAMEMPLLDALSREFRRMTGVTLERENW
QWEQVPDHLKMTFRVVDEHNRKLLEGKDLTALKAQLKDKVQETLSKVADDGLEQSGLHIW
SFGDLPRSYEQKRGSYQVKAWPALVDEKESVAIRLFDSEQEQQKMMWRGQRRLLLLNVPS
PVKYLHEKLPNKAKLGLYFNPYGKVLELIDDCIACGIDKLIGEAGGPAWDQGAFEQLRDK
VRGELNETVVTIAQQVEQILTAVFNINKRLKGRVDMTMALALSDIKAQVNGLVYRGFVTG
NGWQRLADTLRYLNGIERRLEKLPADPHSDRARMLKVQAVEQAYQAWRNKLPPQRQDDEE
VQNIRWMIEELRISYFAQQLGTPYPISDKRILQTMEQIAA