Protein Info for IAI47_08825 in Pantoea sp. MT58

Annotation: tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 232 transmembrane" amino acids 82 to 97 (16 residues), see Phobius details TIGR03725: tRNA threonylcarbamoyl adenosine modification protein YeaZ" amino acids 5 to 221 (217 residues), 214.3 bits, see alignment E=9.7e-68 PF00814: TsaD" amino acids 30 to 152 (123 residues), 97.3 bits, see alignment E=6.5e-32

Best Hits

Swiss-Prot: 70% identical to TSAB_SALTY: tRNA threonylcarbamoyladenosine biosynthesis protein TsaB (tsaB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K14742, hypothetical protease [EC: 3.4.-.-] (inferred from 96% identity to pva:Pvag_1577)

MetaCyc: 67% identical to N6-L-threonylcarbamoyladenine synthase, TsaB subunit (Escherichia coli K-12 substr. MG1655)
RXN-14570 [EC: 2.3.1.234]

Predicted SEED Role

"TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-

Use Curated BLAST to search for 2.3.1.234 or 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (232 amino acids)

>IAI47_08825 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB (Pantoea sp. MT58)
MSARILALDTATEACSAALLNQQQIDARFEIAPRDHTQRILPLVEELLLAQQLELTELDA
LAFGRGPGSFTGVRIGIGIAQGLALGAALPMIAVSTLATLAEGAWRLHGATRVLTAIDAR
MGEVYWAEYQRDEHGAWQGEASESVLSPEAALARMQSLSGEWMTAGTGWQAYPGLQQGHE
LTLIASEVTLPAAQDMLPLAILALAQGNTLLPETAEPTYLRNEVAWKKLPGR